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{{Main|Alpha-fetoprotein}}
[[Alpha-fetoprotein]] (AFP) is significantly expressed in foetal liver. However, the mechanism that led to the suppression of AFP synthesis in adults is not fully known. Exposure of the liver to cancer-causing agents and arrest of liver maturation in childhood can lead to the rise in AFP. AFP can reach until 400–500 μg/L in [[hepatocellular carcinoma]]. AFP concentration of more than 400 μg/L is associated with greater tumour size, involvement of both lobes of liver, portal vein invasion and a lower median survival rate.<ref name="Shivaraj 2009"/>
===Coagulation test===
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