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=== Mitotic Selection ===
Mitotic selection is a drug-free procedure for the selection of mitotic cells from a monolayer undergoing exponential growth.<ref>{{Cite web|url=https://www.biology-online.org/dictionary/Mitotic_cell_selection|title=Mitotic cell selection|date=3 October 2005|website=Biology Online Dictionary|access-date=15 December 2018|archive-date=2 April 2019|archive-url=https://web.archive.org/web/20190402073007/https://www.biology-online.org/dictionary/Mitotic_cell_selection|url-status=dead}}</ref> During mitosis, cells undergo changes in morphology, and mitotic selection takes advantage of this in adherent cells grown in a [[monolayer]]. The cells become more spherical, decreasing the surface area of cell membrane attached to the culture plate. Mitotic cells can therefore be completely detached by gently shaking and collected from the [[Precipitation (chemistry)|supernatant]].<ref name=":0" />
===Nutrient/Serum Deprivation===
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