Push–pull perfusion: Difference between revisions

Revision as of 10:09, 14 May 2024

Revision as of 19:12, 14 May 2024

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this technique replaced the [[cortical cup]] technique for observing neurotransmitters.

In order to analyze concentrations of [[Analyte|analytes]] such as neurotransmitters, a probe consisting of two concentric tubes areis implanted in the region of interest. A pump then pushes a neutral fluid such as [[Saline (medicine)|saline]] or [[Ringer's solution]] through one of the pumpstubes, while another pump extracts the fluid through the other tube. While outside the tubes, the perfusion fluid picks up physiological substances such as neurotransmitters that are present in the area. The concentration of analytes of interest can then be measured in the expelled fluid, indicating in which concentration they are present at the site of interest at any given time.<ref>{{Citation |last=Westerink |first=B.H.C. |title=Microdialysis compared with other in vivo release models |date=1991 |work=Microdialysis in the Neurosciences |pages=23–43 |url=https://doi.org/10.1016/B978-0-444-81194-3.50007-1 |access-date=2024-05-14 |publisher=Elsevier |doi=10.1016/b978-0-444-81194-3.50007-1 |last2=Justice |first2=J.B.}}</ref>

The advent of concentric [[microdialysis]] probes in the 1980s resulted in push-pull sampling falling out of favor, as such probes require less monitoring, and are less invasive than the higher flow rate push-pull probes (>10&nbsp;microliter/min), which could result in lesions if flow is unbalanced.<ref>{{cite journal|last=Myers|first=R.D.|author2=Adell, A. |author3=Lankford, M.F. |title=Simultaneous comparison of cerebral dialysis and push-pull perfusion in the brain of rats: a critical review|journal=Neuroscience & Biobehavioral Reviews|year=1998|volume=22|issue=3|pages=371–387|doi=10.1016/S0149-7634(97)00025-0 |pmid=9579326|s2cid=36994607}}</ref>