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In [[DC Animated Universe|animated continuity]], the first Ace (voiced by [[Hynden Walch]]) was a young girl. Her power is mind control, but she can also create illusions, and drive people insane just by looking at them. She had been experimented on by what appeared to be a clandestine government operation to increase her power. This Ace, along with her Royal Flush Gang has fought [[Batman]] and the [[Justice League]] under the leadership/manipulation of the [[Joker (comics)|Joker]]. Ace first appears in the ''Justice League'' Season 2 episodes ''Wild Cards #1 & 2''. In the episode, The Joker "hosts" a reality TV show with the Justice League pitted against the first Royal Flush gang, attempting to defuse bombs in real time and on television all over Las Vegas. The Joker attempts to use a reluctant Ace to turn the television audience insane after the Justice League wins, but his his effort is narrowly avoided due to Batman's interference.
[[Image:EpilogueJLUrevised.jpg|thumb|250px|right|Ace spends her final moments with Batman]]
Later, under Ace's leadership, a second (or possibly third according to ''[[Batman Beyond]]''-era [[Amanda Waller]]) Royal Flush Gang fought the JLU again. In the Justice League Unlimited episode "Epilogue", Ace's powers have advanced to the point where she can create and alter reality, not just perception. She created a new version of the gang seemingly out of desperation to have friends to play with. Unfortunately, the people Ace empowered decided to go out and commit crimes instead.
As the League battled the Gang (minus Ace), Amanda Waller explained that Ace was dying, and the possible psychic backlash caused by her death could kill millions. (Waller's presence and knowledge indicates that she may have been an early experiment of [[Project Cadmus]]. Batman volunteered to kill her with a special weapon supplied by Waller, though [[Hawkgirl (animated)|Hawkgirl]] wanted to do so at first. When he got to Ace however, it was revealed that Batman had no intentions of killing her.
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