Expression vector: Difference between revisions

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Plant viruses may be used as vectors since the ''Agrobacterium'' method does not work for all plants. Examples of plant virus used are the [[tobacco mosaic virus]] (TMV), [[potato virus X]], and [[cowpea mosaic virus]].<ref>{{cite journal |title= Use of viral vectors for vaccine production in plants |author1=M Carmen Cañizares |author2=Liz Nicholson |author3=George P Lomonossoff |journal=Immunology and Cell Biology |year=2005 |volume=83 |issue=3 |pages= 263–270 |doi=10.1111/j.1440-1711.2005.01339.x |pmid=15877604 |pmc=7165799 }}</ref> The protein may be expressed as a fusion to the coat protein of the virus and is displayed on the surface of assembled viral particles, or as an unfused protein that accumulates within the plant. Expression in plant using plant vectors is often constitutive,<ref>{{cite web |title=How Do You Make A Transgenic Plant? |url=http://cls.casa.colostate.edu/transgeniccrops/how.html |work=Department of Soil and Crop Sciences at Colorado State University |access-date=2013-02-06 |archive-date=2013-01-21 |archive-url=https://web.archive.org/web/20130121061854/http://cls.casa.colostate.edu/TransgenicCrops/how.html |url-status=dead }}</ref> and a commonly used constitutive promoter in plant expression vectors is the [[cauliflower mosaic virus]] (CaMV) 35S promoter.<ref>{{cite journal |author1=Fütterer J. |author2=Bonneville J. M. |author3=Hohn T |title=Cauliflower mosaic virus as a gene expression vector for plants |journal=Physiologia Plantarum
|volume = 79 |issue = 1 |pages = 154–157 |date= May 1990 |doi= 10.1111/j.1399-3054.1990.tb05878.x |bibcode=1990PPlan..79..154F }}</ref><ref>{{cite journal |title=The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants |vauthors=Benfey PN, Chua NH |journal=Science |year=1990 |volume=250|issue=4983 |pages=959–66 |pmid=17746920 |url=http://www.sciencemag.org/site/feature/data/plants2001/PDFs/250-4983-959.pdf |doi=10.1126/science.250.4983.959|bibcode=1990Sci...250..959B |s2cid=35471862 }}</ref>
 
===Mammalian===