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'''Microplate Readers''' (also known as ''Plate readers'') are laboratory instruments designed to detect [[biology|biological]], [[chemistry|chemical]] or [[physics|physical]] events
Sample reactions can be (assayed) in 6-1536 well format microtiter plates. In most cases, a high-intensity lamp passes light to the microtiter well and the light emitted by the reaction happening in the microlpate well is quantified by a detector. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. The first microplate readers availble were filter based while modern day readers are tunable(monochromator based) enabling use of any flurophore and chromophore, allowing assay flexibility as needed in the laboratory. Current day plate reader come with software tools for data analysis, automation, GxP tools, and [[LIMS]] capabilities.
What is Microplate Detection used for?
These devices typically use optical and/or computer vision techniques to evaluate the contents of the microtiter plate wells. An [[ELISA]] plate reader, for example, is used to measure the intensity of the colors formed in each wells. [[ELISPOT]] plate readers are used to count the colored spots that are formed in the course of ELISPOT assays. Using these plate readers can eliminate (or at least, help reduce) the amount of human subjectivity which goes into evaluating the plate contents.▼
ELISAs (Enzyme-Linked ImmunoSorbent Assay, a biochemical technique used mainly in immunology to detect antibodies or antigens)
Protein and cell growth assays
Nucleic acid quantitation
Molecular interactions
Enzyme activity
Cell toxicity, proliferation, and viability
ATP quantification
Immunoassays
Highthroughput screening of compounds and targets in Drug Discovery such as FLIPR assays
More...
▲These devices typically use optical and/or
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