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* for DNA-edited cells, the process may take an extended period of time
==Chemical
The addition of [[exogenous]] substrates can be used to block cells in certain phases of the cell cycle and frequently target [[cell cycle checkpoint]]s. These techniques can be carried out ''[[in vitro]]'' and do not require removal from the culture environment. The most common type of chemical blockade is arrest-and-release, which involves treatment of a culture with a chemical block and subsequent release by washing or addition of a neutralizing agent for the block. While chemical blockade is typically more effective and precise than physical separation, some methods can be imperfect for various reasons, including:
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