Plate reader: Difference between revisions

Revision as of 05:50, 4 May 2007

Revision as of 17:29, 5 May 2007

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'''Microplate Readers''' (also known as ''Plate readers'') are laboratory instruments designed to detect [[biology|biological]], [[chemistry|chemical]] or [[physics|physical]] events of samples in [[microtiter plate]]s.

Sample reactions can be (assayed) in 6-1536 well format microtiter plates. In most cases, a high-intensity lamp passes light to the microtiter well and the light emitted by the reaction happening in the microlpatemicroplate well is quantified by a detector. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. The first microplate readers availbleavailable were filter based while modern day readers are tunable(monochromator based) enabling use of any flurophorefluorophore and chromophore, allowing assay flexibility as needed in the laboratory. Current day plate readerreaders come with software tools for data analysis, automation, GxP tools, and [[LIMS]] capabilities.

What is Microplate Detection used for?

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More...

These devices typically use optical and/or in a few cases lable free techniques to evaluate the contents of the microtiter plate wells. An [[ELISA]] plate reader, for example, is used to measure the intensity of the color formed in each well. [[ELISPOT]] plate readers are used to count the colored spots that are formed in the course of ELISPOT assays. Using these plate readers can eliminate (or at least, help reduce) the amount of human subjectivity which goes into evaluating the plate contents. Microplate readers are widely used in research, drug discovery, bioassay validation, QC and manufacturing processes in the pharmaceticlepharmaceutical and biotechnologybiotechnological industry and academic organizations.

{{LaboratoryEquipment}}