Plate reader: Difference between revisions

Revision as of 17:29, 5 May 2007

Revision as of 21:15, 5 May 2007

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What is Microplate Detection used for?

*ELISAs (Enzyme-Linked ImmunoSorbent Assay, a biochemical technique used mainly in immunology to detect antibodies or antigens)

*Protein and cell growth assays

*Nucleic acid quantitation

*Molecular interactions

*Enzyme activity

*Cell toxicity, proliferation, and viability

*ATP quantification

*Immunoassays

Highthroughput*High throughput screening of compounds and targets in Drug Discovery such as FLIPR assays

More...

These devices typically use optical and/or in a few cases lable free techniques to evaluate the contents of the microtiter plate wells. An [[ELISA]] plate reader, for example, is used to measure the intensity of the color formed in each well. [[ELISPOT]] plate readers are used to count the colored spots that are formed in the course of ELISPOT assays. Using these plate readers can eliminate (or at least, help reduce) the amount of human subjectivity which goes into evaluating the plate contents. Microplate readers are widely used in research, drug discovery, bioassay validation, QC and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.