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Temperature serves as a critical environmental factor in plant tissue culture systems, with optimal incubation temperatures varying significantly among species based on their natural habitat requirements. While 25°C represents the standard incubation temperature suitable for many plant species in vitro, species-specific temperature adaptations should be considered to maximize organogenic potential.{{cn|date=June 2025}}
Geophytic species from temperate regions typically require lower temperature regimes than the standard protocol. Notable examples include bulbous plants such as ''Galanthus'' (snowdrop) which exhibits optimal growth at approximately 15°C, while certain cultivars of ''[[Narcissus (plant)|Narcissus]]'' (daffodil) and ''Allium'' (ornamental onion) demonstrate enhanced regeneration efficiency at around 18°C.{{cn|date=June 2025}}
Conversely, species of tropical origin generally require elevated temperatures for optimal growth and organogenesis in culture. Date palm cultures thrive at 27°C, while ''Monstera deliciosa'' (Swiss cheese plant) exhibits peak regenerative performance at 30°C. These temperature requirements reflect evolutionary adaptations to the plants' native environmental conditions.{{cn|date=June 2025}}
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