Automated Imaging Microscope System: Difference between revisions

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The '''Automated Imaging Microscope System''' (AIMS) was developed by scientists at the [[Aging Research Centre]] (ARC) and the [[University of California, Berkeley]]. [[Steven A. Garan]], was the lead scientists that developed the AIMS system along with Warren Freitag and Jason Neudorf, and many journals articles have been published about the system and the results that it produced. Since the completion of the first version in 1998, newer versions were developed, with the final version being completed in 2006. Empowering investigators to accurately count specific cell populations is essential to all fields of neurobiology. While computer assisted counting technology has been in use for over a decade, advances in an Automated Imaging Microscope System (AIMS), now insure 97% accuracy when comparing computer counts to human counts for both nuclear and cytoplasmic stained tissue. More importantly, regional analysis can now be customized so that only cell populations within specified anatomic regions will be targeted for counting, thus reducing the background noise of non-immunoreactive cells when characterizing specific cell populations. This application was recently used to successfully map the density and distribution of both nuclear expressed estrogen receptor-alpha and cytoplasmicly expressed IGF-1 receptor in specific hypothalamic nuclei. Furthermore, AIMS can now detect intra-hypothalamic differences in receptor expression and measure phenomenon such as lateralization. By using this technology, the evaluation of tissue-level biology can be used to establish neuroendocrine biomarkers of aging, and analyze the neuroendocrine effects of caloric restriction and gene knockout models that extend the lifespan.

==Publications==

*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6J-4KGG1P4-10B&_user=10&_handle=C-WA-A-VD-VD-MsSAYWB-UUW-U-U-VD-U-U-AADUEEDBAV-AAZYCDYAAV-WUBCCYBZA-VD-U&_fmt=full&_coverDate=02%2F28%2F2007&_rdoc=4528&_orig=browse&_srch=%23toc%235032%232007%23999579998%23639966!&_cdi=5032&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=d5741475a812c1d5a283114bba5379a82e1993c5025659810f6ce66fd3135f4f Tracking"Novel changesmethods in hypothalamic IGFcomputer-1assisted sensitivitytissue withanalysis: agingCustomized regional targeting of both cytoplasmic and caloricnuclear-stained restrictiontissue"]. ''Experimental Gerontology'', Volume 42, Issues 1-2 , January-February 2007, Pages 11141-12.]142

*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6J-4KGG1P4-B10&_user=10&_handle=C-WA-A-VD-VD-MsSAYWB-UUW-U-U-VD-U-U-AADUEEDBAV-AAZYCDYAAV-WUBCCYBZA-VD-U&_fmt=full&_coverDate=02%2F28%2F2007&_rdoc=2845&_orig=browse&_srch=%23toc%235032%232007%23999579998%23639966!&_cdi=5032&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=2e1993c5025659810f6ce66fd3135f4fd5741475a812c1d5a283114bba5379a8 Novel"Tracking methodschanges in computerhypothalamic IGF-assisted1 tissuesensitivity analysis:with Customized regional targeting of both cytoplasmicaging and nuclear-stainedcaloric tissuerestriction"]. ''Experimental Gerontology'', Volume 42, Issues 1-2 , January-February 2007, Pages 14111-142]12.

* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=1599072117194562&query_hl=3&itool=pubmed_docsum Caloric"Insulin-like restrictiongrowth reducesfactor-1 cellreceptor lossimmunoreactive andcells maintainsare estrogenselectively receptor-alpha immunoreactivitymaintained in the pre-opticparaventricular hypothalamus of femalecalorically B6D2F1restricted mice,"]. Neuro''Int EndocrinolJ LettDev Neurosci.'' 20052007 JunFeb;2625(31):19723-203. ]8

*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6J-4KGG1P4-10&_user=10&_handle=C-WA-A-VD-VD-MsSAYWB-UUW-U-U-VD-U-U-AADUEEDBAV-AAZYCDYAAV-WUBCCYBZA-VD-U&_fmt=full&_coverDate=02%2F28%2F2007&_rdoc=45&_orig=browse&_srch=%23toc%235032%232007%23999579998%23639966!&_cdi=5032&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=d5741475a812c1d5a283114bba5379a8 Tracking changes in hypothalamic IGF-1 sensitivity with aging and caloric restriction. Experimental Gerontology, Volume 42, Issues 1-2 , January-February 2007, Pages 11-12.]

*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=1719456217034982&query_hl=3&itool=pubmed_docsum Insulin"Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells are selectively maintained in the paraventricularsupraoptic hypothalamus ofis reduced in calorically restricted mice"]. ''Int J Dev Neurosci.'' 20072006 FebNovember;2524(17):23431-8]6.

* [http://www.ncbi.nlmfasebj.nih.govorg/entrezcgi/query.fcgicontent/meeting_abstract/20/4/A536?dbmaxtoshow=pubmed&cmdHITS=Retrieve10&dopthits=AbstractPlus10&list_uidsRESULTFORMAT=17034982&query_hlFIRSTINDEX=30&itoolvolume=pubmed_docsum20&issue=4&resourcetype=HWCIT Age-dependent"A lossstudy of insulin-like growth factor-1I receptor immunoreactive cellsimmunoreactivity in the supraoptic hypothalamusnucleus isof reducedyoung inand caloricallyold restrictedfemale B6D2F1 mice"]. Int''FASEB JJournal'', Dev Neurosci.April 2006, Nov;24(7):431-6357.]5

* [http://www.fasebj.org/cgi/content/meeting_abstract/20/4/A536?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&FIRSTINDEX=0&volume=20&issue=4&resourcetype=HWCIT A study of insulin-like growth factor-I receptor immunoreactivity in the supraoptic nucleus of young and old female B6D2F1 mice. FASEB Journal, April 2006, 357.5]

* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15990721&query_hl=3&itool=pubmed_docsum Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice, Neuro Endocrinol Lett. 2005 Jun;26(3):197-203. ]

* [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T6J-4DK6DF0-1-1&_cdi=5032&_user=10&_orig=browse&_coverDate=11%2F01%2F2004&_sk=999609988&view=c&wchp=dGLbVzz-zSkzV&md5=3b4c858a77ad92f1e24cbcf3c26e895e&ie=/sdarticle.pdf A survey of estrogen receptor-alpha immunoreactivity in the hypothalamus of young, old, and old-calorie restricted female B6D2F1 mice, Experimental Gerontology, Volume 39, Issues 11-12, November-December 2004, Page 1771]

* [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T6J-4DK6DF0-1-1&_cdi=5032&_user=10&_orig=browse&_coverDate=11%2F01%2F2004&_sk=999609988&view=c&wchp=dGLzVlz-zSkWW&md5=3b4c858a77ad92f1e24cbcf3c26e895e&ie=/sdarticle.pdf Protocal for higher resolution histological images of the mammalian brain, Experimental Gerontology, Volume 39, Issues 11-12, November-December 2004, Page 1770]

* [http://www.linuxjournal.com/article/3459 Automated Imaging Microscope System, Linux Journal 2000, Issue 70, Februrary, Page 32-35]

* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15990721&query_hl=3&itool=pubmed_docsum "Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice"], ''Neuro Endocrinol Lett.'' 2005 June;26(3):197-203.

* [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T6J-4DK6DF0-1-1&_cdi=5032&_user=10&_orig=browse&_coverDate=11%2F01%2F2004&_sk=999609988&view=c&wchp=dGLbVzz-zSkzV&md5=3b4c858a77ad92f1e24cbcf3c26e895e&ie=/sdarticle.pdf "A survey of estrogen receptor-alpha immunoreactivity in the hypothalamus of young, old, and old-calorie restricted female B6D2F1 mice"], ''Experimental Gerontology'', Volume 39, Issues 11-12, November-December 2004, Page 1771]

* [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T6J-4DK6DF0-1-1&_cdi=5032&_user=10&_orig=browse&_coverDate=11%2F01%2F2004&_sk=999609988&view=c&wchp=dGLzVlz-zSkWW&md5=3b4c858a77ad92f1e24cbcf3c26e895e&ie=/sdarticle.pdf Protocal"Protocol for higher resolution histological images of the mammalian brain"], ''Experimental Gerontology'', Volume 39, Issues 11-12, November-December 2004, Page 1770]

* [http://www.linuxjournal.com/article/3459 "Automated Imaging Microscope System"], ''[[Linux Journal]]'' 2000, Issue 70, FebruraryFebruary, Page 32-35]

==See also==

* [[Aging Research Centre]]

== External links ==

* Aging Research Centre: http://www.arclab.org

* Automated Imaging Microscope System (AIMS) http://www.arclab.org/AIMS/

[[Category:Microscopes]]