Plate reader: Difference between revisions

Revision as of 15:34, 12 March 2008

Revision as of 22:06, 20 June 2008

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Sample reactions can be (assayed) in 6-1536 well format microtiter plates. In most cases, a high-intensity lamp passes light to the microtiter well and the light emitted by the reaction happening in the microplate well is quantified by a detector. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. The first microplate readers available were filter based while modern day readers are tunable(monochromator based) enabling use of any fluorophore and chromophore, allowing assay flexibility as needed in the laboratory. Current day plate readers come with software tools for data analysis, automation, GxP tools, and [[LIMS]] capabilities.

What is Microplate Detection may used for?:

*[[ELISA]]s

*ELISAs (Enzyme-Linked ImmunoSorbent Assay, a biochemical technique used mainly in immunology to detect antibodies or antigens)

*Protein and cell growth assays

*Nucleic acid quantitation