Microarray analysis techniques: Difference between revisions

Revision as of 22:49, 4 November 2008

Revision as of 10:20, 2 April 2009

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==Background correction==

Depending on the type of array, signal related to nonspecific binding of the fluorophore can be subtracted to achieve better results. One approach involves subtracting the average

signal intensity of the area between spots. A variety of tools for background correction and further analysis are available from TIGR,<ref>{{cite web |url=http://www.tigr.org/software/microarray.shtml |title=J. Craig Venter Institute -- Software |accessdate=2008-01-01 |format= |work=}}</ref> and, Agilent (GeneSpring).<ref>{{cite web |url=http://www.chem.agilent.com/scripts/pds.asp?lpage=27881 |title=Agilent | GeneSpring GX |accessdate=2008-01-02 |format= |work=}}</ref> and Ocimum Biosolutions (Genowiz).<ref>{{cite web |url=http://www.ocimumbio.com/web/bioinformatic/prod_details.asp?prod_id=20&prodType=1 |title=Ocimum Biosolutions | Genowiz |accessdate=2009-04-02 |format= |work=}}</ref>

==Quality control==