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[[Image:Yeast colonies array 1536 format.jpg|thumb|300px|Arrayed yeast showing synthetic lethal interactions]]
Synthetic Genetic Array analysis was initially developed by Tong et al. <ref> A. H. Tong et al., Science 294, 2364 (2001)16.</ref> in 2001 and has since been used by many groups working in a wide range of biomedical fields. SGA utilizes the entire genome yeast knock-out set created by the yeast genome deletion project <ref> http://sequence-www.stanford.edu/group yeast_deletion_project/deletions3.html</ref>.
Synthetic Genetic Array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in ''S.cerevisae'', the query gene is crossed systematically with a deletion mutant array (DMA) containing every viable knockout [[Open reading frame|ORF]] of the yeast genome (currently 4786 strains)<ref>http://www.openbiosystems.com/GeneExpression/Yeast/YKO/ </ref>. The resulting [[diploids]] are then sporulated by transferring to a media containing reduced nitrogen. The [[haploid]] progeny are then put through a series of selection platings and incubations to select for double mutants. The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.
[[Image:Pinning robot.jpg|thumb|280px|right|Replicating yeast colonies during SGA analysis using a pinning robot]]
Due to the large number of precise replication steps in SGA analysis, robots are widely used to perform the colony manipulations. There are a few systems specifically designed for SGA analysis which greatly decrease the time to analyse a query gene. Generally these have a series of pins which are used to transfer cells to and from plates, with one system utilizing disposable pads of pins to eliminate washing cycles.
*[[tetrad (genetics)]]
*[[Two hybrid screening|Yeast two-hybrid]]
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