SSC buffer: Difference between revisions

Revision as of 05:00, 13 May 2009

Revision as of 07:46, 1 September 2009

Line 3:

{{context}}

In [[biochemistry]] and [[molecular biology]], the '''saline-sodium citrate (SSC) buffer''' is used as a hybridisation [[buffer (chemistry)|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ hybridisation]], [[DNA Microarray]] or [[Northern blotting]].

TheA 20×20X stock solution consists of 3 [[molar (concentration)|M]] [[sodium chloride]] and 300 [[milli-|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid|HCl]]).

==See also==