G-CSF factor stem-loop destabilising element: Difference between revisions

Revision as of 14:13, 26 June 2009

Revision as of 17:04, 11 March 2010

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{{orphan|date=January 2008}}

{{Rfam_box|acc=RF00183| description=G-CSF factor stem-loop destabilising element (SLDE) |abbreviation=G-CSF_SLDE |avg_length=93.9080 |avg_identity=8483.00 |type=Cis-reg; |se={{PMID|11865046}} |ss=Published; {{PMID|11865046}}; |release=910.10}}

The '''G-CSF factor stem-loop destabilising element (SLDE)''' is an [[cis-regulatory element|RNA element]] secreted by [[fibroblast]]s and endothelial cells in response to the inflammatory mediators [[interleukin-1]] (IL-1) and tumour necrosis factor alpha and by activated macrophages. The synthesis of G-CSF is regulated both transcriptionally and through control of [[mRNA]] stability. In unstimulated cells G-CSF mRNA is unstable but becomes stabilised in response to IL-1 or tumour necrosis factor alpha, and also in the case of monocytes and macrophages, in response to lipopolysaccharide. It is likely that the presence of the SLDE in the G-CSF mRNA contributes to the specificity of regulation of G-CSF mRNA and enhances the rate of shortening of the poly(A) tail.<ref>{{cite journal | last = Putland | first = RA | coauthors = Sassinis TA, Harvey JS, Diamond P, Coles LS, Brown CY, Goodall GJ | year = 2002 | title = RNA destabilization by the granulocyte colony-stimulating factor stem-loop destabilizing element involves a single stem-loop that promotes deadenylation | journal = Mol Cell Biol | volume = 22 | pages = 1664&ndash;1673 | pmid = 11865046 | doi = 10.1128/MCB.22.6.1664-1673.2002}}</ref>