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Line 1: {{Article issues\|orphan =February 2009\|notability =June 2008\|context =October 2009}} In [[biochemistry]] and [[molecular biology]], the '''saline-sodium citrate (SSC) buffer''' is used as a hybridisation [[buffer (chemistry)\|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ ~~hybridisation~~hybridization]], [[DNA Microarray]] or [[Northern blotting]]. A 20X stock solution consists of 3 [[molar (concentration)\|M]] [[sodium chloride]] and 300 [[milli-\|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid\|HCl]]).

SSC buffer: Difference between revisions