Selector technique: Difference between revisions

Revision as of 05:45, 18 August 2009

Revision as of 19:32, 7 July 2010

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{{Orphan|date=February 2009}}

The '''selector technique''' allows multiplex amplification of arbitrary sets of genomic sequences. Genomic [[DNA]] is digested with restriction enzymes, circularized by [[hybridisation (molecular biology)|hybridisation]] to selectors and subsequently attached to a vector sequence by [[ligation]]. The procedure results in circular [[DNA]] [[molecules]] with an included general primer pair motif that can be used for amplification by [[PCR]] or RCA.

===The selector construct===

A selector consists of two oligonucleotides, one '''Vector''' oligonucletide and one '''Selector probe'''. Together they form one '''Selector''' with target specific ends on each side of a general primer motif.

===Selection mechanisms===

* (I) A selector probe hybridizes with both ends of the selected target.

* (II) A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using [[Taq polymerase]].

===Publications===

*[http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method]

*[http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments]

{{DEFAULTSORT:Selector-Technique}}

[[Category:DNA]]