Since it's completion genome sequencing has become more accurate, less expensive, and quicker.
==Overview==
TheThere promiseare ofnow the [[Human genome project]] was that determining the human [[genetic code]] would lead to great advances in understanding human disease and to developing drugs and other therapies that were engineered tomany interactsequenced withgenomes specificwaiting biologicalfor processesstudy. However, it has become clear that understandingthe data of the DNA sequence is not enough. The [[human genome]] encodes between 40,000 and 100,000 proteins[[protein]]s (the [[proteome]]), and justin asorder importantto asmake understandinguse of the [[DNA]] sequence, [[protein sequencessequence]]s ismust understandingbe theirpredicted and verified, [[Protein structure]] prediction|three-dimensionalpredicted structure]]and verified, howand [[protein epxressioninteraction]] is regulated,predicted and how proteins interactverified.
Many methods exist to experimentally determine protein-protein interactions, including [[Affinity chromatography]], yeast [[two-hybrid screening]] techniques, [[fluorescence resonance energy transfer]] (FRET), and Surface Plasmon Resonance (SPR). However, with at least 40,000 unique protein sequences potentially available in every cell, the number of popssiblepossible interactions becomes so enormous that physical testing can only be performed on a small subset of the most interesting proteins at grate expense. Protein-protein interaction prediction seeks to use advanced computational methods to rapidly investigate many possible interactions in order to identify those with the greatest potential for exerimentalexperimental investigation. <!--potential for what? Needs to talk about drug discovery etc.-->
Knowledge is the first step Understanding the second and helping the third.
PPIP provides a means of gaining knowledge of how [[organism]]s work.
understanding how the inner workings of organisms makes it possible to develop a method of fixing organisms when broken and helping them resist attack
