G-CSF factor stem-loop destabilising element: Difference between revisions

Revision as of 14:59, 7 July 2011

Revision as of 10:35, 17 October 2011

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{{Infobox rfam

{{Rfam_box|acc=RF00183| description=G-CSF factor stem-loop destabilising element (SLDE) |abbreviation=G-CSF_SLDE |avg_length=93.80 |avg_identity=83.00 |type=Cis-reg; |se={{PMID|11865046}} |ss=Published; {{PMID|11865046}} |release=10.0}}

| Name = G-CSF factor stem-loop destabilising element (SLDE)

| image = RF00183.jpg

| width =

| caption = Predicted [[secondary structure]] and [[sequence conservation]] of G-CSF_SLDE

| Symbol = G-CSF_SLDE

| AltSymbols =

| Rfam = RF00183

| miRBase =

| miRBase_family =

| RNA_type = [[Cis-regulatory element | Cis-reg]]

| Tax_domain = [[Eukaryota]]

| GO =

| SO = {{SO|0000233}}

| CAS_number =

| EntrezGene =

| HGNCid =

| OMIM =

| PDB =

| RefSeq =

| Chromosome =

| Arm =

| Band =

| LocusSupplementaryData =

}}

The '''G-CSF factor stem-loop destabilising element (SLDE)''' is an [[cis-regulatory element|RNA element]] secreted by [[fibroblast]]s and endothelial cells in response to the inflammatory mediators [[interleukin-1]] (IL-1) and [[tumour necrosis factor-alpha]] and by activated [[macrophage]]s. The synthesis of G-CSF is regulated both [[transcription (genetics)|transcriptionally]] and through control of [[mRNA]] stability. In unstimulated cells G-CSF mRNA is unstable but becomes stabilised in response to IL-1 or tumour necrosis factor alpha, and also in the case of [[monocyte]]s and macrophages, in response to [[lipopolysaccharide]]. It is likely that the presence of the SLDE in the G-CSF mRNA contributes to the specificity of regulation of G-CSF mRNA and enhances the rate of shortening of the [[polyadenylation|poly(A) tail]].<ref>{{cite journal | last = Putland | first = RA | coauthors = Sassinis TA, Harvey JS, Diamond P, Coles LS, Brown CY, Goodall GJ | year = 2002 | title = RNA destabilization by the granulocyte colony-stimulating factor stem-loop destabilizing element involves a single stem-loop that promotes deadenylation | journal = Mol Cell Biol | volume = 22 | pages = 1664&ndash;1673 | pmid = 11865046 | doi = 10.1128/MCB.22.6.1664-1673.2002 | issue = 6 | pmc = 135610}}</ref>