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===Preparation===
Specimen preparation is critical for evaluating the tumor in the automated system. In the first part of the preparation process the biopsied tissue is cut to an appropriate size (typically 4 mm), fixed in buffered [[formalin]], dehydrated in ethanol-[[xylene]], embedded in [[Paraffin wax|paraffin]], [[thin section]]ed typically to 4 um slices, then moutned onto at least two [[barcode]]d slides (a [[scientific control|control]] and a test). Next the paraffin is removed from the tissue, the tissue is rehydrated, then [[staining|stained]]. Any inconsistency in these procedures from case to case may result in uncertainties in the outcome of the analysis. These potential and irreducible inconsistencies in analysis results motivated the development of Automated Tissue Image Systems.
===Acquisition===
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