CIT Program Tumor Identity Cards: Difference between revisions

Revision as of 13:56, 30 May 2013

Revision as of 14:14, 30 May 2013

Line 29:

=== Biochip Experiments ===

The CIT program chooses commercial technologies and efficient instruments to carry out the hybridizations on biochips (especilly [http://www.affymetrix.com/estore/browse/products.jsp?productId=131455&categoryId=35760&productName=GeneChip-Human-Genome-U133-Plus-2.0-Array#1_1 Affymetrix GeneChip arrays] to study the expression of genes, [http://www.illumina.com/technology/beadarray_technology.ilmn Illumina BeadChip arraysBeadArrays] to study allelotypes, SNPs and [http://www.illumina.com/applications/epigenetics/array-based_methylation_analysis.ilmn methylome], and next-generation sequencing of exomes and [http://www.illumina.com/applications/epigenetics/small_rna_analysis.ilmn miRNAs]). This increases the yield of the platforms and the reproducibility of the results. Standardized protocols are set up at all stages of the process (preparation of the samples, hybridization, scanning, image analysis etc.). A key objective of the CIT program is to process the greatest possible number of samples on the same platform to minimize bias related to the experiments, to optimize the parameters of the different stages, and to enable comprehensive quality control checks.

Between January 2004 and January 2013, over '''14,000 biochip experiments''' were carried out.

=== Analysis & Validation ===