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4.) The measurements saved for each particle are then used to generate image population statistics, or as inputs to algorithms for filtering and sorting the particles into groups of similar types. In some systems, sophisticated [[pattern recognition]] techniques may also be employed in order to separate different particle types contained in a heterogeneous sample.
Imaging particle analyzers can be subdivided into two distinct types, static and dynamic. While the basic principles are the same, the methods of image acquisition are different in nature, and each has advantages and disadvantages.
Static image acquisition is the most common form. Almost all microscopes can be easily adapted to accept a digital camera via a [[C mount]] adaptor. This type of set-up is often referred to as a [[digital microscope]], although many systems using that name are used only for displaying an image on a [[Video monitor#video display|monitor]].
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The major drawback to static image acquisition is that it is time consuming, both in sample preparation (getting the sample onto the slide with proper dilution if necessary), and in multiple movements of the stage in order to be able to acquire a statistically significant number of particles to count/measure. Computer-controlled X-Y positioning stages are sometimes used in these systems to speed the process up and to reduce the amount of operator intervention, but it is still a time consuming process, and the motorized stages can be expensive due to the level of precision required when working at high magnification.
[[File:Basic flow through diag on white.png|thumb|Diagram showing flow-through architecture for dynamic imaging particle anaysis.]]In Dynamic image acquisition, large amounts of sample are imaged by moving the sample past the microscope optics and using [[flash (photography)#high speed flash|high speed flash]] illumination to effectively "freeze" the motion of the sample. The flash is [[synchronization|
== References ==
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