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[[File:Basic flow through diag on white.png|thumb|Diagram showing flow-through architecture for dynamic imaging particle anaysis.]]In Dynamic image acquisition, large amounts of sample are imaged by moving the sample past the microscope optics and using [[flash (photography)#high speed flash|high speed flash]] illumination to effectively "freeze" the motion of the sample. The flash is [[synchronization|synchronized]] with a high [[shutter speed]] in the camera to further prevent motion blur.
The flow cell is characterized by its depth perpendicular to the optical axis. In order to keep the particles in focus, the flow depth is restricted so that the particles remain in a plane of best focus perpendicular to the optical axis. This is similar in concept to the effect of the microscope slide plus cover slip in a static imaging system. Since depth of field decreases exponentially with increasing magnification, the depth of the flow cell must be narrowed significantly with higher magnifications. [[File:Flow cell Cross Section.png|thumb|Flow cell Cross Section|Diagram showing the flow cell cross-section perpendicular to the optical axis in a dynamic imaging particle analyzer.]].
== References ==
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