Imaging particle analysis: Difference between revisions

Revision as of 19:48, 18 October 2013

Revision as of 19:49, 18 October 2013

Line 47:

===Dynamic Image Acquisition:===

[[File:Basic flow through diag on white.png|thumb|Diagram showing flow-through architecture for dynamic imaging particle anaysis.]]In Dynamic image acquisition, large amounts of sample are imaged by moving the sample past the microscope optics and using [[flash (photography)#highHigh speed flash|Highhigh speed flash]] illumination to effectively "freeze" the motion of the sample. The flash is [[synchronization|synchronized]] with a high [[shutter speed]] in the camera to further prevent motion blur.

[[File:Flow cell Cross Section.png|thumb|Flow cell Cross Section|Diagram showing the flow cell cross-section perpendicular to the optical axis in a dynamic imaging particle analyzer.]]The flow cell is characterized by its depth perpendicular to the optical axis. In order to keep the particles in focus, the flow depth is restricted so that the particles remain in a plane of best focus perpendicular to the optical axis. This is similar in concept to the effect of the microscope slide plus cover slip in a static imaging system. Since depth of field decreases exponentially with increasing magnification, the depth of the flow cell must be narrowed significantly with higher magnifications.