Cell-free protein array: Difference between revisions

Revision as of 05:51, 23 January 2014

Revision as of 23:49, 5 February 2014

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[[Image:Figure 5 DAPA.png|thumb|600px|'''Figure 5: Schematic diagram of DAPA''']]

DNA array to protein array (DAPA) is a method developed in 2007 to repeatedly produce protein arrays by ‘printing’ them from a single DNA template array, on demand<ref>He, M., O. Stoevesandt, et al. (2008). "Printing protein arrays from DNA arrays." Nat Methods 5(2): 175–7.</ref> (Figure 5). It starts with the spotting and immobilization of an array of DNA templates onto a glass slide. The slide is then assembled face-to-face with a second slide pre-coated with a protein-capturing reagent, and a membrane soaked with cell extract is placed between the two slides for transcription and translation to take place. The newly- synthesized his-tagged proteins are then immobilized onto the slide to form the array. In the publication in 18 of 20 replications a protein microarray copy could be generated. Potentially the process can be repeated as often as needed, as long as the DNA is unharmed by DNAses, degradation or mechanical abrasion.

==Advantages==