Restriction fragment length polymorphism: Difference between revisions

Revision as of 21:13, 27 June 2014

Revision as of 21:21, 27 June 2014

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[[File:RFLPDemo2.gif|right|thumb|450px|<center>Schematic for RFLP by VNTR length variation.</center>]]

=kgigigig== Examples ===

There are two common mechanisms by which the size of a particular restriction fragment can vary. In the first schematic, a small segment of the genome is being detected by a DNA probe (thicker line). In allele "A", the genome is cleaved by a restriction enzyme at three nearby sites (triangles), but only the rightmost fragment will be detected by the probe. In allele "a", restriction site 2 has been lost by a [[genetic mutation|mutation]], so the probe now detects the larger fused fragment running from sites 1 to 3. The second diagram shows how this fragment size variation would look on a Southern blot, and how each allele (two per individual) might be inherited in members of a family.