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==Steps in plant transformation==
# Propagate binary vector in ''E. coli''
# Isolate binary vector from ''E.coli'' and engineer (introduce a foreign gene)
# Re-introduce engineered binary vector into ''E. coli'' to amplify
# Isolate engineered binary vector and introduce into ''Agrobacteria'' containing a modified (relatively small) Ti plasmid
# Infect plant tissue with engineered ''Agrobacteria'' (T-DNA containing the foreign gene gets inserted into a plant cell genome)
# In each cell T-DNA gets integrated at a different site in the genome
* Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.
=== Consequences of the insertion ===
# Foreign DNA inserted
# Insertional mutagenesis (but not lethal for the plant cell – as the organism is diploid)
=== Problem ===
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