Upstream activating sequence: Difference between revisions

Revision as of 03:02, 15 September 2014

Revision as of 03:03, 15 September 2014

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==Instances==

Several studies have been conducted with ''Saccharomyces cerevisiae'' to explore the exact function of upstream activation sequences, often focusing on the GAL1-GAL10 intergenic region. One such study explored the galactose-responsive upstream activation sequence (UAS{{sub|G}}),looking at the influence of proximity to this UAS for nucleosome positioning. The role of specific regions of UAS{{sub|G}} was analyzed by inserting oligonucleotides with different binding properties, leading to the successful identification of a region responsible for the creation of an ordered array.<ref>{{cite journal|last1=Fedor|first1=Martha J.|last2=Lue|first2=Neal F.|last3=Kornberg|first3=Roger D.|title=Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast|journal=Journal of Molecular Biology|date=5 November 1988|volume=204|issue=1|pages=109-127}}</ref> AdditionalAnother studiesstudy have been done to looklooked at the effect of inserting the UAS{{sub|G}} into the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (GPD). When compared to the native GPD promoter, the transcriptional activity remained equivalently enhanced under induced conditions.<ref>{{cite journal|last1=Bitter|first1=Grant A.|last2=Egan|first2=Kevin M.|title=Expression of interferon-gamma from hybrid yeast GPD promoters containing upsream regulatory sequences from the GAL1-GAL10 intergenic region|journal=Gene|date=30 September 1988|volume=69|issue=2|pages=193-207}}</ref>

It has been shown to be involved in five of the seven genes which are glucose repressed. These genes were repressed in yeast ([[Saccharomyces cerevisiae]]) eventually after growing for hours in YPD (Yeast peptone dextrose) media. After using up glucose for fermentation, these genes get activated for aerobic respiration.