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===''GAL1-GAL10'' Intergenic Region===
Several studies have been conducted with [[''Saccharomyces cerevisiae'']] to explore the exact function of upstream activation sequences, often focusing on the ''GAL1-GAL10'' intergenic region [https://www.wikigenes.org/e/gene/e/852307.html]. Its property to bind the ''GAL4'' protein is utilised in the [[GAL4/UAS system|GAL4/UAS technique]] for controlled gene misexpression in Drosophila. In this technique, four related binding sites between the ''GAL10'' and ''GAL1'' loci in ''Saccharomyces cerevisiae'' serve as an Upstream Activating Sequences (UAS) element through ''GAL4'' binding.<ref>{{cite journal|last1=Duffy|first1=Joseph B.|title=GAL4 system in Drosophilia: A Fly Geneticist's Swiss Army Knife|journal=Genesis|date=2002|volume=34|issue=1-2|pages=1-15}}</ref>.
One study explored the galactose-responsive upstream activation sequence (UAS{{sub|G}}),looking at the influence of proximity to this UAS for nucleosome positioning. The role of specific regions of UAS{{sub|G}} was analyzed by inserting oligonucleotides with different binding properties, leading to the successful identification of a region responsible for the creation of an ordered array.<ref>{{cite journal|last1=Fedor|first1=Martha J.|last2=Lue|first2=Neal F.|last3=Kornberg|first3=Roger D.|title=Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast|journal=Journal of Molecular Biology|date=5 November 1988|volume=204|issue=1|pages=109-127}}</ref>.
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