Upstream activating sequence: Difference between revisions

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==Examples of Upstream Activation Sequences in Research==

===''GAL1-GAL10'' Intergenic Region (UAS{{sub|G}})===

The property of the ''GAL1-GAL10'' to bind the ''GAL4'' protein is utilised in the [[GAL4/UAS system|GAL4/UAS technique]] for controlled gene misexpression in Drosophila. This is the most popular form of binary expression in ''Drosophila melanogaster'', a system which has been adapted for many uses to make ''Drosophila melanogaster'' one of the most genetically tractable multicellular organisms. <ref>{{cite journal|last1=Wimmer|first1=Ernst A.|title=Applications of insect transgenesis|journal=Nature Reviews Genetics|date=March 2003|volume=4|pages=225-232|doi=10.1038/nrg1021}}</ref> In this technique, four related binding sites between the ''GAL10'' and ''GAL1'' loci in ''Saccharomyces cerevisiae'' serve as an Upstream Activating Sequences (UAS) element through ''GAL4'' binding.<ref>{{cite journal|last1=Duffy|first1=Joseph B.|title=GAL4 system in Drosophilia: A Fly Geneticist's Swiss Army Knife|journal=Genesis|date=2002|volume=34|issue=1-2|pages=1-15}}</ref>. Several studies have been conducted with [[Saccharomyces cerevisiae|''Saccharomyces cerevisiae'']] to explore the exact function of upstream activation sequences, often focusing on the aforementioned ''GAL1-GAL10'' intergenic region [https://www.wikigenes.org/e/gene/e/852307.html].