Bio-layer interferometry: Difference between revisions

Revision as of 14:40, 16 October 2013

Revision as of 14:56, 20 November 2014

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[[File:Bio-layer interferometry wavelength shift due to analyte binding .gif|thumb|Figure 3]]

'''Bio-Layer Interferometryinterferometry''' is a label-free technology for measuring biomolecular interactions within the [[interactome]].<ref>Current Biosensor Technologies in Drug Discovery. Cooper, M. A. Drug Discovery World, 2006, 68-82</ref><ref>Higher-throughput, label-free, real-time molecular interaction analysis. Rich, R. L.; Myszka, D. G. Analytical Biochemistry, 2007, 361, 1-6</ref> It is an optical analytical technique that analyzes the [[Interference (wave propagation)|interference]] pattern of white light reflected from two surfaces: a layer of immobilized [[protein]] on the biosensor tip, and an internal reference layer (Figure 1). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real-time (Figures 1 and 2).

The binding between a ligand immobilized on the biosensor tip surface and an analyte in solution produces an increase in [[optical thickness]] at the biosensor tip, which results in a [[wavelength]] shift, Δλ (Figure 3), which is a direct measure of the change in thickness of the biological layer. Interactions are measured in real time, providing the ability to monitor binding specificity, rates of association and dissociation, or concentration, with precision and accuracy.