Microprocessor complex subunit DGCR8: Difference between revisions

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'''Pasha''', also known as '''DGCR8''' in vertebrates, is a [[protein]] localized to the [[cell nucleus]] that is required for [[microRNA]] processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to [[miRNA]] fragments by the enzyme [[Dicer]]. Pasha contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.

'''DCGR8''' (DiGeorge syndrome chromosomal region 8) is a [[protein]] that in humans is encoded by the ''DCGR8'' [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=54487| accessdate = }}</ref> In other animals, particularly the common [[model organism]]s ''[[Drosophila melanogaster]]'' and ''[[Caenorhabditis elegans]]'', the protein is known as ''Pasha'' (partner of [[Drosha]]).<ref>{{cite journal|last1=Denli|first1=AM|last2=Tops|first2=BB|last3=Plasterk|first3=RH|last4=Ketting|first4=RF|last5=Hannon|first5=GJ|title=Processing of primary microRNAs by the Microprocessor complex.|journal=Nature|date=11 November 2004|volume=432|issue=7014|pages=231-5|pmid=15531879}}</ref> It is a required component of the [[RNA interference]] pathway.

'''Pasha''',The also known as '''DGCR8''' in vertebrates,protein is a [[protein]] localized to the [[cell nucleus]] thatand is required for [[microRNA]] (miRNA) processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to [[miRNA]] fragments by the enzyme [[Dicer]]. Pasha contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.<ref>{{cite journal | pmid=16963499 | year=2006 | last1=Yeom | first1=KH | last2=Lee | first2=Y | last3=Han | first3=J | last4=Suh | first4=MR | last5=Kim | first5=VN | title=Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | volume=34 | issue=16 | pages=4622–9 | doi=10.1093/nar/gkl458 | pmc=1636349 | journal=Nucleic acids research}}</ref>

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==References==

{{reflist}}

* {{cite journal | pmid=16963499 | year=2006 | last1=Yeom | first1=KH | last2=Lee | first2=Y | last3=Han | first3=J | last4=Suh | first4=MR | last5=Kim | first5=VN | title=Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | volume=34 | issue=16 | pages=4622–9 | doi=10.1093/nar/gkl458 | pmc=1636349 | journal=Nucleic acids research}}

==Further reading==

[[Category:Proteins]]

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[[Category:MicroRNA]]

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*{{cite journal | author=Maruyama K, Sugano S |title=Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. |journal=Gene |volume=138 |issue= 1-2 |pages= 171–4 |year= 1994 |pmid= 8125298 |doi=10.1016/0378-1119(94)90802-8 }}

*{{cite journal | author=Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, ''et al.'' |title=Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. |journal=Gene |volume=200 |issue= 1-2 |pages= 149–56 |year= 1997 |pmid= 9373149 |doi=10.1016/S0378-1119(97)00411-3 }}

*{{cite journal | author=Hartley JL, Temple GF, Brasch MA |title=DNA cloning using in vitro site-specific recombination. |journal=Genome Res. |volume=10 |issue= 11 |pages= 1788–95 |year= 2001 |pmid= 11076863 |doi=10.1101/gr.143000 | pmc=310948 }}

*{{cite journal | author=Simpson JC, Wellenreuther R, Poustka A, ''et al.'' |title=Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. |journal=EMBO Rep. |volume=1 |issue= 3 |pages= 287–92 |year= 2001 |pmid= 11256614 |doi= 10.1093/embo-reports/kvd058 | pmc=1083732 }}

*{{cite journal | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 }}

*{{cite journal | author=Shiohama A, Sasaki T, Noda S, ''et al.'' |title=Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. |journal=Biochem. Biophys. Res. Commun. |volume=304 |issue= 1 |pages= 184–90 |year= 2003 |pmid= 12705904 |doi=10.1016/S0006-291X(03)00554-0 }}

*{{cite journal | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}

*{{cite journal | author=Collins JE, Wright CL, Edwards CA, ''et al.'' |title=A genome annotation-driven approach to cloning the human ORFeome. |journal=Genome Biol. |volume=5 |issue= 10 |pages= R84 |year= 2005 |pmid= 15461802 |doi= 10.1186/gb-2004-5-10-r84 | pmc=545604 }}

*{{cite journal | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 }}

*{{cite journal | author=Wiemann S, Arlt D, Huber W, ''et al.'' |title=From ORFeome to biology: a functional genomics pipeline. |journal=Genome Res. |volume=14 |issue= 10B |pages= 2136–44 |year= 2004 |pmid= 15489336 |doi= 10.1101/gr.2576704 | pmc=528930 }}

*{{cite journal | author=Gregory RI, Yan KP, Amuthan G, ''et al.'' |title=The Microprocessor complex mediates the genesis of microRNAs. |journal=Nature |volume=432 |issue= 7014 |pages= 235–40 |year= 2004 |pmid= 15531877 |doi= 10.1038/nature03120 }}

*{{cite journal | author=Han J, Lee Y, Yeom KH, ''et al.'' |title=The Drosha-DGCR8 complex in primary microRNA processing. |journal=Genes Dev. |volume=18 |issue= 24 |pages= 3016–27 |year= 2005 |pmid= 15574589 |doi= 10.1101/gad.1262504 | pmc=535913 }}

*{{cite journal | author=Landthaler M, Yalcin A, Tuschl T |title=The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. |journal=Curr. Biol. |volume=14 |issue= 23 |pages= 2162–7 |year= 2005 |pmid= 15589161 |doi= 10.1016/j.cub.2004.11.001 }}

*{{cite journal | author=Mehrle A, Rosenfelder H, Schupp I, ''et al.'' |title=The LIFEdb database in 2006. |journal=Nucleic Acids Res. |volume=34 |issue= Database issue |pages= D415–8 |year= 2006 |pmid= 16381901 |doi= 10.1093/nar/gkj139 | pmc=1347501 }}

*{{cite journal | author=Han J, Lee Y, Yeom KH, ''et al.'' |title=Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. |journal=Cell |volume=125 |issue= 5 |pages= 887–901 |year= 2006 |pmid= 16751099 |doi= 10.1016/j.cell.2006.03.043 }}

*{{cite journal | author=Faller M, Matsunaga M, Yin S, ''et al.'' |title=Heme is involved in microRNA processing. |journal=Nat. Struct. Mol. Biol. |volume=14 |issue= 1 |pages= 23–9 |year= 2007 |pmid= 17159994 |doi= 10.1038/nsmb1182 }}

*{{cite journal | author=Sohn SY, Bae WJ, Kim JJ, ''et al.'' |title=Crystal structure of human DGCR8 core. |journal=Nat. Struct. Mol. Biol. |volume=14 |issue= 9 |pages= 847–53 |year= 2007 |pmid= 17704815 |doi= 10.1038/nsmb1294 }}

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{{refend}}

{{PDB Gallery|geneid=54487}}

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[[Category:Proteins]]

[[Category:MicroRNA]]