Microprocessor complex subunit DGCR8: Difference between revisions

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{{PBB|geneid=54487}}

'''DCGR8DGCR8''' (DiGeorge syndrome chromosomal region 8) is a [[protein]] that in humans is encoded by the ''DCGR8DGCR8'' [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=54487| accessdate = }}</ref> In other animals, particularly the common [[model organism]]s ''[[Drosophila melanogaster]]'' and ''[[Caenorhabditis elegans]]'', the protein is known as ''Pasha'' (partner of [[Drosha]]).<ref>{{cite journal|last1=Denli|first1=AM|last2=Tops|first2=BB|last3=Plasterk|first3=RH|last4=Ketting|first4=RF|last5=Hannon|first5=GJ|title=Processing of primary microRNAs by the Microprocessor complex.|journal=Nature|date=11 November 2004|volume=432|issue=7014|pages=231-5|pmid=15531879}}</ref> It is a required component of the [[RNA interference]] pathway.

The protein is localized to the [[cell nucleus]] and is required for [[microRNA]] (miRNA) processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme [[Dicer]]. Pasha contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.<ref>{{cite journal | pmid=16963499 | year=2006 | last1=Yeom | first1=KH | last2=Lee | first2=Y | last3=Han | first3=J | last4=Suh | first4=MR | last5=Kim | first5=VN | title=Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | volume=34 | issue=16 | pages=4622–9 | doi=10.1093/nar/gkl458 | pmc=1636349 | journal=Nucleic acids research}}</ref>