Microprocessor complex subunit DGCR8: Difference between revisions

Revision as of 07:18, 15 February 2015

Revision as of 07:25, 15 February 2015

Line 1:

{{PBB|geneid=54487}}

'''DGCR8''' (DiGeorge syndrome chromosomal region 8) is a [[protein]] that in humans is encoded by the ''DGCR8'' [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=54487| accessdate = }}</ref> In other animals, particularly the common [[model organism]]s ''[[Drosophila melanogaster]]'' and ''[[Caenorhabditis elegans]]'', the protein is known as ''Pasha'' (partner of [[Drosha]]).<ref>{{citevcite2 journal |last1 vauthors = Denli|first1= AM|last2=, Tops|first2= BB|last3=, Plasterk|first3= RH|last4=, Ketting|first4= RF|last5=, Hannon|first5= GJ | title = Processing of primary microRNAs by the Microprocessor complex. | journal =Nature|date=11 NovemberNature 2004| volume = 432 | issue = 7014 | pages = 231-5 | date = Nov 2004 | pmid = 15531879 | doi = 10.1038/nature03049 }}</ref> It is a required component of the [[RNA interference]] pathway.

== Function ==

The protein is localized to the [[cell nucleus]] and is required for [[microRNA]] (miRNA) processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme [[Dicer]]. Pasha contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.<ref>{{cite journal | pmid=16963499 | year=2006 | last1=Yeom | first1=KH | last2=Lee | first2=Y | last3=Han | first3=J | last4=Suh | first4=MR | last5=Kim | first5=VN | title=Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | volume=34 | issue=16 | pages=4622–9 | doi=10.1093/nar/gkl458 | pmc=1636349 | journal=Nucleic acids research}}</ref>

The protein is localized to the [[cell nucleus]] and is required for [[microRNA]] (miRNA) processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme [[Dicer]]. Pasha contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.<ref>{{citevcite2 journal | pmid=16963499vauthors | year=2006 | last1=Yeom | first1=KH, | last2=Lee | first2=Y, | last3=Han | first3=J, | last4=Suh | first4=MR, | last5=Kim | first5=VN | title = Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | journal = Nucleic Acids Research | volume = 34 | issue = 16 | pages = 4622–9 | doiyear =10.1093/nar/gkl458 2006 | pmid = 16963499 | pmc = 1636349 | journaldoi =Nucleic acids10.1093/nar/gkl458 research}}</ref>

<!-- The PBB_Summary template is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->

{{PBB_Summary

| section_title =

| summary_text =

}}

== References ==

{{reflist}}

== Further reading ==

{{refbegin | 2}}

* {{vcite2 journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1-2 | pages = 171–4 | year = 1994 | date = Jan 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}

{{PBB_Further_reading

* {{vcite2 journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1-2 | pages = 149–56 | year = 1997 | date = Oct 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}

| citations =

* {{citevcite2 journal | authorvauthors =Maruyama KHartley JL, SuganoTemple SGF, Brasch MA | title =Oligo-capping: aDNA simplecloning methodusing toin replacevitro thesite-specific caprecombination structure| ofjournal eukaryotic= mRNAsGenome with oligoribonucleotides.Research |journal=Gene |volume =138 10 | issue = 1-211 | pages = 171–41788–95 | year = 19942001 | date = Nov 2000 | pmid = 812529811076863 | pmc = 310948 | doi = 10.10161101/0378-1119(94)90802-8 gr.143000 }}

* {{citevcite2 journal | authorvauthors =Suzuki YSimpson JC, Yoshitomo-NakagawaWellenreuther KR, MaruyamaPoustka KA, ''etPepperkok al.''R, Wiemann S | title =Construction andSystematic subcellular characterizationlocalization of anovel fullproteins length-enrichedidentified andby a 5'large-end-enrichedscale cDNA library.sequencing | journal =Gene EMBO Reports | volume =200 1 | issue = 1-23 | pages = 149–56287–92 | year = 19972001 | date = Sep 2000 | pmid = 937314911256614 | pmc = 1083732 | doi = 10.10161093/S0378embo-1119(97)00411-3 reports/kvd058 }}

* {{citevcite2 journal | authorvauthors =Hartley JLShiohama A, TempleSasaki GFT, BraschNoda MAS, Minoshima S, Shimizu N | title =DNA Molecular cloning usingand expression analysis of a novel gene DGCR8 located in vitrothe site-specificDiGeorge recombination.syndrome chromosomal region | journal =Genome Res.Biochemical and Biophysical Research Communications | volume =10 304 | issue = 111 | pages = 1788–95184–90 | year = 20012003 |pmid date = 11076863Apr 2003 |doi pmid =10.1101/gr.143000 12705904 | pmcdoi =310948 10.1016/S0006-291X(03)00554-0 }}

* {{citevcite2 journal | authorvauthors =Simpson JCCollins JE, WellenreutherWright RCL, PoustkaEdwards ACA, ''etDavis al.''MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I | title =Systematic subcellularA localizationgenome ofannotation-driven novelapproach proteinsto identifiedcloning bythe large-scalehuman cDNAORFeome sequencing.| |journal =EMBO Rep.Genome Biology | volume =1 5 | issue = 310 | pages = 287–92R84 | year = 20012005 | pmid = 1125661415461802 | pmc = 545604 | doi = 10.10931186/embogb-reports/kvd058 | pmc=10837322004-5-10-r84 }}

* {{citevcite2 journal | authorvauthors =Strausberg RLWiemann S, FeingoldArlt EAD, GrouseHuber LHW, ''etWellenreuther al.''R, |title=GenerationSchleeger andS, initialMehrle analysisA, ofBechtel moreS, thanSauermann 15M,000 full-lengthKorf humanU, andPepperkok mouseR, cDNASültmann sequences.H, Poustka A |journal title =Proc. Natl.From Acad.ORFeome Sci.to U.S.A.biology: a functional genomics pipeline | journal = Genome Research | volume =99 14 | issue = 2610B | pages = 16899–9032136–44 | year = 20032004 | date = Oct 2004 | pmid = 1247793215489336 | pmc = 528930 | doi = 10.10731101/pnasgr.242603899 | pmc=1392412576704 }}

* {{citevcite2 journal | authorvauthors =Shiohama AGregory RI, SasakiYan TKP, NodaAmuthan SG, ''etChendrimada al.''T, |title=MolecularDoratotaj cloningB, andCooch expressionN, analysisShiekhattar ofR a| noveltitle gene= DGCR8The locatedMicroprocessor incomplex mediates the DiGeorgegenesis syndromeof chromosomalmicroRNAs region.| |journal =Biochem. Biophys. Res. Commun.Nature | volume =304 432 | issue = 17014 | pages = 184–90235–40 | year = 20032004 | date = Nov 2004 | pmid = 1270590415531877 | doi = 10.10161038/S0006-291X(03)00554-0 nature03120 }}

* {{citevcite2 journal | authorvauthors =Ota THan J, SuzukiLee Y, NishikawaYeom TKH, ''etKim al.''YK, Jin H, Kim VN | title =Complete sequencingThe andDrosha-DGCR8 characterizationcomplex ofin 21,243primary full-lengthmicroRNA human cDNAs.processing | journal =Nat. Genet.Genes & Development | volume =36 18 | issue = 124 | pages = 40–53016–27 | year = 2005 | date = Dec 2004 | pmid = 1470203915574589 | pmc = 535913 | doi = 10.10381101/ng1285gad.1262504 }}

* {{citevcite2 journal | authorvauthors =Collins JE,Landthaler Wright CLM, EdwardsYalcin CAA, ''etTuschl al.''T | title =A genomeThe annotation-drivenhuman approachDiGeorge tosyndrome cloningcritical theregion humangene ORFeome8 and Its D. melanogaster homolog are required for miRNA biogenesis | journal =Genome Biol.Current Biology : CB | volume =5 14 | issue = 1023 | pages = R842162–7 | year = 2005 | date = Dec 2004 | pmid = 1546180215589161 | doi = 10.11861016/gb-j.cub.2004-5-10-r84 | pmc=545604.11.001 }}

* {{citevcite2 journal | authorvauthors =Gerhard DSMehrle A, WagnerRosenfelder LH, FeingoldSchupp EAI, ''etdel al.''Val |title=TheC, statusArlt D, qualityHahne F, andBechtel expansionS, ofSimpson theJ, NIHHofmann full-lengthO, cDNAHide project:W, theGlatting MammalianKH, GeneHuber CollectionW, (MGC).Pepperkok R, Poustka A, Wiemann S | title = The LIFEdb database in 2006 | journal =Genome Res.Nucleic Acids Research | volume =14 34 | issue = 10BDatabase issue | pages = 2121–7D415–8 | year = 20042006 |pmid date = 15489334Jan 2006 |doi= 10.1101/gr.2596504pmid = 16381901 | pmc =528928 1347501 | doi = 10.1093/nar/gkj139 }}

* {{citevcite2 journal | authorvauthors =Wiemann SHan J, ArltLee DY, HuberYeom WKH, ''etNam al.''JW, Heo I, Rhee JK, Sohn SY, Cho Y, Zhang BT, Kim VN | title =From ORFeomeMolecular tobasis biology:for athe functionalrecognition genomicsof pipeline.primary microRNAs by the Drosha-DGCR8 complex | journal =Genome Res.Cell | volume =14 125 | issue = 10B5 | pages = 2136–44887–901 | year = 20042006 | date = Jun 2006 | pmid = 1548933616751099 | doi = 10.11011016/grj.2576704 | pmc=528930cell.2006.03.043 }}

* {{citevcite2 journal | authorvauthors =Gregory RIFaller M, YanMatsunaga KPM, AmuthanYin GS, ''etLoo al.''JA, Guo F | title =The MicroprocessorHeme complexis mediatesinvolved thein genesismicroRNA of microRNAs.processing | journal = Nature Structural & Molecular Biology | volume =432 14 | issue = 70141 | pages = 235–4023–9 | year = 20042007 | date = Jan 2007 | pmid = 1553187717159994 | doi = 10.1038/nature03120nsmb1182 }}

* {{citevcite2 journal | authorvauthors =Han JSohn SY, LeeBae YWJ, Kim JJ, Yeom KH, ''etKim al.''VN, Cho Y | title =The Drosha-DGCR8Crystal complexstructure inof primaryhuman microRNADGCR8 processing.core | journal =Genes Dev.Nature Structural & Molecular Biology | volume =18 14 | issue = 249 | pages = 3016–27847–53 | year = 20052007 | date = Sep 2007 | pmid = 1557458917704815 | doi = 10.11011038/gad.1262504 | pmc=535913nsmb1294 }}

*{{cite journal | author=Landthaler M, Yalcin A, Tuschl T |title=The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. |journal=Curr. Biol. |volume=14 |issue= 23 |pages= 2162–7 |year= 2005 |pmid= 15589161 |doi= 10.1016/j.cub.2004.11.001 }}

*{{cite journal | author=Mehrle A, Rosenfelder H, Schupp I, ''et al.'' |title=The LIFEdb database in 2006. |journal=Nucleic Acids Res. |volume=34 |issue= Database issue |pages= D415–8 |year= 2006 |pmid= 16381901 |doi= 10.1093/nar/gkj139 | pmc=1347501 }}

*{{cite journal | author=Han J, Lee Y, Yeom KH, ''et al.'' |title=Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. |journal=Cell |volume=125 |issue= 5 |pages= 887–901 |year= 2006 |pmid= 16751099 |doi= 10.1016/j.cell.2006.03.043 }}

*{{cite journal | author=Faller M, Matsunaga M, Yin S, ''et al.'' |title=Heme is involved in microRNA processing. |journal=Nat. Struct. Mol. Biol. |volume=14 |issue= 1 |pages= 23–9 |year= 2007 |pmid= 17159994 |doi= 10.1038/nsmb1182 }}

*{{cite journal | author=Sohn SY, Bae WJ, Kim JJ, ''et al.'' |title=Crystal structure of human DGCR8 core. |journal=Nat. Struct. Mol. Biol. |volume=14 |issue= 9 |pages= 847–53 |year= 2007 |pmid= 17704815 |doi= 10.1038/nsmb1294 }}

}}

{{refend}}

{{PDB Gallery|geneid=54487}}

<!-- The PBB_Controls template provides controls for Protein Box Bot, please see Template:PBB_Controls for details. -->

{{PBB_Controls

| update_page = yes

| require_manual_inspection = no

| update_protein_box = yes

| update_summary = yes

| update_citations = yes

}}

{{gene-22-stub}}