Microprocessor complex subunit DGCR8: Difference between revisions

Revision as of 07:25, 15 February 2015

Revision as of 20:10, 15 February 2015

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{{PBB|geneid=54487}}

The '''DGCR8''' microprocessor complex subunit ''(DiGeorge syndrome chromosomal'' [or ''critical''] ''region 8)'' is a [[protein]] that in humans is encoded by the ''DGCR8'' [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=54487| accessdate = }}</ref> In other animals, particularly the common [[model organism]]s ''[[Drosophila melanogaster]]'' and ''[[Caenorhabditis elegans]]'', the protein is known as ''Pasha'' (partner of [[Drosha]]).<ref>{{vcite2 journal | vauthors = Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ | title = Processing of primary microRNAs by the Microprocessor complex | journal = Nature | volume = 432 | issue = 7014 | pages = 231-5 | date = Nov 2004 | pmid = 15531879 | doi = 10.1038/nature03049 }}</ref> It is a required component of the [[RNA interference]] pathway.

== Function ==

The proteinDCGR8 is localized to the [[cell nucleus]] and is required for [[microRNA]] (miRNA) processing. It binds to [[Drosha]], an [[RNase III]] [[enzyme]], to form the ''Microprocessor complex'' that cleaves a [[primary transcript]] known as pri-miRNA to a characteristic [[stem-loop]] structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme [[Dicer]]. PashaDCGR8 contains an [[RNA]]-binding ___domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.<ref>{{vcite2 journal | vauthors = Yeom KH, Lee Y, Han J, Suh MR, Kim VN | title = Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing | journal = Nucleic Acids Research | volume = 34 | issue = 16 | pages = 4622–9 | year = 2006 | pmid = 16963499 | pmc = 1636349 | doi = 10.1093/nar/gkl458 }}</ref>

== References ==

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== Further reading ==

{{refbegin | 2}}

* {{vcite2 journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1-2 | pages = 171–4 | year = 1994 | date = Jan 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}

* {{vcite2 journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1-2 | pages = 149–56 | year = 1997 | date = Oct 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}

* {{vcite2 journal | vauthors = Hartley JL, Temple GF, Brasch MA | title = DNA cloning using in vitro site-specific recombination | journal = Genome Research | volume = 10 | issue = 11 | pages = 1788–95 | year = 2001 | date = Nov 2000 | pmid = 11076863 | pmc = 310948 | doi = 10.1101/gr.143000 }}

* {{vcite2 journal | vauthors = Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S | title = Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing | journal = EMBO Reports | volume = 1 | issue = 3 | pages = 287–92 | year = 2001 | date = Sep 2000 | pmid = 11256614 | pmc = 1083732 | doi = 10.1093/embo-reports/kvd058 }}

* {{vcite2 journal | vauthors = Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N | title = Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region | journal = Biochemical and Biophysical Research Communications | volume = 304 | issue = 1 | pages = 184–90 | year = 2003 | date = Apr 2003 | pmid = 12705904 | doi = 10.1016/S0006-291X(03)00554-0 }}

* {{vcite2 journal | vauthors = Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I | title = A genome annotation-driven approach to cloning the human ORFeome | journal = Genome Biology | volume = 5 | issue = 10 | pages = R84 | year = 2005 | pmid = 15461802 | pmc = 545604 | doi = 10.1186/gb-2004-5-10-r84 }}

* {{vcite2 journal | vauthors = Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A | title = From ORFeome to biology: a functional genomics pipeline | journal = Genome Research | volume = 14 | issue = 10B | pages = 2136–44 | year = 2004 | date = Oct 2004 | pmid = 15489336 | pmc = 528930 | doi = 10.1101/gr.2576704 }}

* {{vcite2 journal | vauthors = Gregory RI, Yan KP, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, Shiekhattar R | title = The Microprocessor complex mediates the genesis of microRNAs | journal = Nature | volume = 432 | issue = 7014 | pages = 235–40 | year = 2004 | date = Nov 2004 | pmid = 15531877 | doi = 10.1038/nature03120 }}

* {{vcite2 journal | vauthors = Han J, Lee Y, Yeom KH, Kim YK, Jin H, Kim VN | title = The Drosha-DGCR8 complex in primary microRNA processing | journal = Genes & Development | volume = 18 | issue = 24 | pages = 3016–27 | year = 2005 | date = Dec 2004 | pmid = 15574589 | pmc = 535913 | doi = 10.1101/gad.1262504 }}

* {{vcite2 journal | vauthors = Landthaler M, Yalcin A, Tuschl T | title = The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis | journal = Current Biology : CB | volume = 14 | issue = 23 | pages = 2162–7 | year = 2005 | date = Dec 2004 | pmid = 15589161 | doi = 10.1016/j.cub.2004.11.001 }}

* {{vcite2 journal | vauthors = Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S | title = The LIFEdb database in 2006 | journal = Nucleic Acids Research | volume = 34 | issue = Database issue | pages = D415–8 | year = 2006 | date = Jan 2006 | pmid = 16381901 | pmc = 1347501 | doi = 10.1093/nar/gkj139 }}

* {{vcite2 journal | vauthors = Han J, Lee Y, Yeom KH, Nam JW, Heo I, Rhee JK, Sohn SY, Cho Y, Zhang BT, Kim VN | title = Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex | journal = Cell | volume = 125 | issue = 5 | pages = 887–901 | year = 2006 | date = Jun 2006 | pmid = 16751099 | doi = 10.1016/j.cell.2006.03.043 }}

* {{vcite2 journal | vauthors = Faller M, Matsunaga M, Yin S, Loo JA, Guo F | title = Heme is involved in microRNA processing | journal = Nature Structural & Molecular Biology | volume = 14 | issue = 1 | pages = 23–9 | year = 2007 | date = Jan 2007 | pmid = 17159994 | doi = 10.1038/nsmb1182 }}

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[[Category:Proteins]]

[[Category:MicroRNA]]