Restriction fragment length polymorphism: Difference between revisions

Revision as of 00:05, 23 July 2006

Revision as of 13:25, 4 August 2006

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==Method==

Usually, [[DNA]] from an individual specimen is first extracted and purified. Purified DNA may be amplified by [[Polymerase Chain Reaction]] (PCR). The DNA is then cut into ''restriction fragments'' by [[restriction enzyme|endonucleases]], which only cut where there are specific [[DNA sequence]]s, termed palindromes, recognized by the enzymes. The restriction fragments are then separated according to length by [[agarose gel electrophoresis]]. The resulting gel may be enhanced by [[Southern blot]]ting.

==Result==