Synthetic genetic array: Difference between revisions

Revision as of 23:59, 27 August 2015

Revision as of 16:52, 7 February 2016

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| last14 = Gross | first14 = C. A.

| title = High-throughput, quantitative analyses of genetic interactions in E. Coli

| journal = Nature methodsMethods

| volume = 5

| issue = 9

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| pmc =

}}</ref>

[[Image:Yeast colonies array 1536 format.jpg|thumb|300px|Arrayed yeast showing synthetic lethal interactions. Synthetic lethal interactions are those pairs of colonies with reduced or no growth.]]

==Background==

Synthetic Genetic Array analysis was initially developed by Tong et al.<ref name="H. Tong 2001"/> in 2001 and has since been used by many groups working in a wide range of biomedical fields. SGA utilizes the entire genome yeast knock-out set created by the yeast genome deletion project.<ref> http://sequence-www.stanford.edu/group yeast_deletion_project/deletions3.html</ref>

==Procedure==

Synthetic Genetic Array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in ''S.cerevisae'', the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout [[Open reading frame|ORF]] of the yeast genome (currently 4786 strains).<ref>http://www.openbiosystems.com/GeneExpression/Yeast/YKO/ </ref> The resulting [[diploids]] are then sporulated by transferring to a media containing reduced nitrogen. The [[haploid]] progeny are then put through a series of selection platings and incubations to select for double mutants. The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.

[[Image:Pinning robot.jpg|thumb|280px|right|Replicating yeast colonies during SGA analysis using a pinning robot]]