Synthetic genetic array: Difference between revisions

Revision as of 09:25, 17 March 2016

Revision as of 20:32, 3 April 2016

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'''Synthetic Geneticgenetic Arrayarray analysis''' ('''SGA''') is a [[high-throughput screening|high-throughput]] technique for exploring [[synthetic lethality|synthetic lethal]] and synthetic sick [[genetic interactions]] ([[Synthetic lethality|SSL]]).<ref name="H. Tong 2001">{{Cite journal

| last1 = Tong | first1 = A. H. Y.

| last2 = Evangelista | first2 = M.

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==Background==

Synthetic Geneticgenetic Arrayarray analysis was initially developed by Tong et al.<ref name="H. Tong 2001"/> in 2001 and has since been used by many groups working in a wide range of biomedical fields. SGA utilizes the entire genome yeast knock-out set created by the yeast genome deletion project.<ref>{{cite web|url=http://sequence-www-sequence.stanford.edu/group /yeast_deletion_project/deletions3.html|title=''Saccharomyces'' Genome Deletion Project|work=}}</ref>

==Procedure==

Synthetic Geneticgenetic Arrayarray analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in ''S.cerevisae'', the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout [[Open reading frame|ORF]] of the yeast genome (currently 4786 strains).<ref>{{cite web|title=Yeast Knockout Strains
|url=http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archiveurl=https://web.archive.org/web/20111119012937/http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archivedate=November 17, 2011|work=Open Biosystems}}</ref> The resulting [[diploids]] are then sporulated by transferring to a media containing reduced nitrogen. The [[haploid]] progeny are then put through a series of selection platings and incubations to select for double mutants. The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.

[[Image:Pinning robot.jpg|thumb|280px|right|Replicating yeast colonies during SGA analysis using a pinning robot]]

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*[[Synthetic lethality]]

*[[Synthetic viability]]

==References==

{{Reflist}}