Random amplification of polymorphic DNA: Difference between revisions

Revision as of 04:40, 12 May 2017

Revision as of 00:10, 22 October 2017

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Selecting the right sequence for the primer is very important because different sequences will produce different band patterns and possibly allow for a more specific recognition of individual strains.

=== Limitations of RAPD ===

* Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish whether a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). Codominant RAPD markers, observed as different-sized DNA segments amplified from the same locus, are detected only rarely.

* PCR is an enzymatic reaction, therefore, the quality and concentration of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible.

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== See also ==

* [[Cleaved amplified polymorphic sequence]]

* [[Genetic marker]]

* [[Cleaved Amplified Polymorphic Sequence]]

==References==

{{Reflist}}