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Due to the large number of precise replication steps in SGA analysis, robots are widely used to perform the colony manipulations. There are a few systems specifically designed for SGA analysis, which greatly decrease the time to analyse a query gene. Generally these have a series of pins which are used to transfer cells to and from plates, with one system utilizing disposable pads of pins to eliminate washing cycles. Computer programs can be used to analyze the colony sizes from images of the plates thus automating the SGA scoring and chemical-genetics profiling.
== Step for a yeast high content genome-wide genetic screening system(SGA golden road map) ==
There are six major components
# Mutant collection
# Material and tools for handling the mutants
# Image analysis system
# Automatic quantification and scoring system
# Confirmation approaches
# Data analysis tools
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==See also==
*[[Tetrad (genetics)]]
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