Synthetic genetic array: Difference between revisions

Revision as of 21:43, 13 February 2019

Revision as of 21:45, 13 February 2019

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However, if you are trying for new, yeast model, you could have to either a genome sequencing and can predict the possible ORF by the good reference yeast genome(For example: with Saccharomyces cerevisiae). Consider a special case: If you don’t have a reference genome, you should go for [[Transcriptomics technologies|transcriptome]] and genome analysis of that new model organism.

* Material and tools for handling the mutants<br />Once you have your mutant library in solid media. If mutants are in solid media, we have arranged the mutants with 1:3 ration, i.e. for one wild type to 3 mutants array(why? Wild type works as an internal control and in a solid media nutrient should not be shared equally for avoiding bias). Once you have single gene deleted mutants, you can start the tools for handling the mutants. In SGA it is referred to as “ Pinning”. ROTOR-HAD versionversions(referred as pinning robot) used for pinning the yeast mutants. This machine installed with a user-friendly interface which helps to pin the samples from sources plates to experimental plates

#Image analysis system