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==== [[Nocodazole]] ====
Nocodazole is a rapidly-reversible inhibitor of [[microtubule]] [[polymerization]] that can be used to arrest cells before [[Anaphase]] at the [[Spindle checkpoint|spindle assembly checkpoint]] in the metaphase/anaphase transition. The microtubule poison works by blocking the formation of the [[Spindle apparatus|mitotic spindles]] that attach to and pull apart [[sister chromatids]] in dividing cells. Cells will remain arrested until the nocodazole has been washed out. Nocodazole does not appear to disrupt interphase metabolism, and released cells return to normal cell cycle progression.<ref>{{Cite journal|last=Zieve|first=Gary W.|last2=Turnbull|first2=Deborah|last3=Mullins|first3=J.Michael|last4=McIntosh|first4=J.Richard|date=April 1980|title=Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor Nocodazole: Nocodazole accumulated mitotic cells|url=|journal=Experimental Cell Research|volume=126|issue=2|pages=397–405|doi=10.1016/0014-4827(80)90279-7|pmid=6153987
==== Inhibition of [[Cyclin-dependent kinase 1|CDK1]] ====
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=== Contact Inhibition ===
Contact inhibition occurs when cells are allowed to grow to high or full confluence, maximizing cell-to-cell contact. This triggers arrest in early G1 in normal cells. Arrest is reversed by replating cells at a lower density.<ref name=":1" /> Because of the proliferation-promoting mutations intrinsic to cancer, tumor cell lines are not usually able to undergo contact inhibition, though there are exceptions.<ref>{{Cite journal|last=Zeng|first=Qi|last2=Hong|first2=Wanjin|date=11 March 2008|title=The Emerging Role of the Hippo Pathway in Cell Contact Inhibition, Organ Size Control, and Cancer Development in Mammals|url=|journal=Cancer Cell|volume=13|issue=3|pages=188–192|doi=10.1016/j.ccr.2008.02.011|pmid=18328423
==External links==
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