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'''Single Particle Extinction and Scattering (SPES)''' is a technique in [[physics]] that is used to characterise micro and [[nanoparticle]]s suspended in a fluid through two independent parameters, the diameter and the [[refractive index|effective refractive index]].<ref>{{Cite journal |title=Single particle extinction and scattering optical method unveils in real time the influence of the blood components on polymeric nanoparticles |journal = Nanomedicine: Nanotechnology, Biology and Medicine|volume = 13|issue = 8|last=Sanvito |first=Tiziano |last2=Bigini |first2=Paolo |date=November 2017
A [[laser]] generates a [[gaussian beam]] which focuses inside a flow cell. A particle that passes through the focal region generates an interference in the beam, which is collected with a sensor. Through this signal it is possible to derive the real part and the imaginary part of the forward scattering field from each particle.
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