Random amplification of polymorphic DNA: Difference between revisions

Revision as of 05:07, 26 August 2019

Revision as of 04:01, 9 September 2019

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=== Limitations ===

* Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish weather a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). Codominant RAPD markers, observed as different-sized DNA segments amplified from the same locus, are detected only rarely.

* PCR is an enzymatic reactoonreaction, therefore, the quality and concentration of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible.

* Mismatches between the primer and the template may result in the total absence of PCR product as well as in a merely decreased amount of the product. Thus, the RAPD results can be difficult to interpret.