Job plot: Difference between revisions

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In this method, the sum of the [[molar concentration]]s of the two binding partners (e.g. a [[protein]] and [[ligand]] or a metal and a ligand) is held constant, but their [[mole fractions]] are varied. An observable that is proportional to complex formation (such as absorption signal or enzymatic activity) is plotted against the mole fractions of these two components.

χ_A is the mole fraction of compound A and P is the physical property being measured to understand complex formation. This property is most oftentimes UV absorbance.<ref name=Renny>Renny, J. S.; Tomasevich, L. L.; Tallmadge, E. H.; Collum, D. B. "Method of Continuous Variations: applications of job plots to the molecular associations in organometallic chemistry. ''Angew Chem Int Ed Engl'' (2013); '''46''', 11998 – 201311998–2013.</ref>

The maximum (or minimum) on the plot corresponds to the stoichiometry of the two species if sufficiently high concentrations are used.<ref>Huang, C.Y. "Determination of Binding Stoichiometry by the Continuous Variation Method: The Job Plot. ''Methods in Enzymology'' (1982); '''87''', 509-525.</ref> The plot also provides insight to understand the equilibrium constant (K_eq) of complex formation. A greater curvature leads to a more evenly distributed equilibrium, while a more triangle-shaped plot signifies a large K_eq.<ref name = "Renny" /> Further, after determining the equilibrium constant, we can determine what complexes (ratio of A and B) are present in solution.<ref name = "Stoichiometry">Facchiano, A. and Ragone, R. "Modification of Job’s method for determining the stoichiometry of protein – protein complexes. ''Analytical Biochemistry'' (2003); '''313''', 170 – 172.</ref> In addition, the peak of the Job Plot corresponds to the mole fraction of ligands bound to a molecule, which is important for studying [[ligand field theory]].<ref>Hauser, A. "Ligand Field Theoretical Considerations. ''Adv Polym Sci'' (2004); '''233''', 49 – 58.</ref> An early work of I. Ostromisslensky describes essentially this approach.<ref>Ostromisslensky, I., ''Berichte der Deutschen Chemischen Gesellschaft'' (1911),; '''44''' (1), 268-273.</ref>

== Requirements ==

There are several conditions that must be met in order for Job's method to be applicable.<ref name="MacCarthy">{{cite journal|last=MacCarthy|first=Patrick|author2=Zachary D. Hill|date=February 1986|title=Novel Approach to Job's Method|journal=Journal of Chemical Education|volume=63|issue=2|pages=162–167|doi=10.1021/ed063p162|bibcode=1986JChEd..63..162H}}</ref> Firstly, the property being studied must vary in direct proportion to the concentration of the species. In the case of UV-visible spectroscopy, for example, this means that the system must conform to the [[Beer–Lambert law|Beer-Lambert law]]. In addition, the total concentration of the two binding partners, the [[pH]] and [[ionic strength]] of the solution must all be maintained at fixed values throughout the experiment.

Finally, there must only be only one complex in solution which predominates over all others under the conditions of the experiment. This requirement means that only systems with high association constants, or systems in which only one stoichiometry can form, are suitable for analysis by Job plot. As such, the use of the Job plot in [[supramolecular chemistry]] has been advised against.<ref>{{Cite journal|last=Brynn Hibbert|first=D.|last2=Thordarson|first2=Pall|date=2016-10-25|title=The death of the Job plot, transparency, open science and online tools, uncertainty estimation methods and other developments in supramolecular chemistry data analysis|url=http://xlink.rsc.org/?DOI=C6CC03888C|journal=Chem. Commun.|language=en|volume=52|issue=87|pages=12792–12805|doi=10.1039/c6cc03888c|issn=1364-548X|pmid=27779264}}</ref>

==References==