Split and pool synthesis: Difference between revisions

Revision as of 14:37, 24 November 2020

Revision as of 13:27, 25 November 2020

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[[File:Flow diagram of split and pool synthesis.png|thumb|left|Flow diagram of the S&P synthesis. Circles: colored BBs, black and white support, divergent arrows: dividing into equal portions, vertical arrows: coupling; convergent arrows: mixing and homogenizing]]

The split and pool synthesis (S&P synthesis) differs from traditional synthetic methods. The important novelty is the use of compound mixtures in the process. This is the reason of its unprecedentedly high productivity. Using the method one single chemist can make more compoundcompounds in a weakweek than all chemists produced in the whole history of chemistry.

The S&P synthesis is applied in a stepwise manner by repeating three operations in each step of the process:

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The split and pool synthesis was first applied to prepare peptide libraries on solid support. The synthesis was realized in a home-made manual device shown in the figure. The device has a tube with 20 holes to which reaction vessels could be attached. One end of the tube is linked to a waste container and a water pump. Left shows loading and filtering, right coupling-shaking position.

In the early years of combinatorial chemistry, an automatic machine was constructed and commercialized at AdvancedChemTech (Louisville KY USA). All operations of the S&P synthesis are carried automatically under computer control. At present, the Titan 357 automatic synthesizer is available at aapptec (Louisville KY, USA).<ref>https://www.peptide.com/peptide-synthesizers/titan/</ref>

==Encoded split and pool synthesis==