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In organic chemistry, the saline-sodium citrate (SSC) buffer is a hybridisation buffer. It is used in molecular biology for washing and to control stringency, for example in Southern blotting or Northern blotting.
The 20× stock solution consists of 3 M sodium chloride and 300 mM trisodium citrate (adjusted to pH 7.0 with HCl).
References
- Material safety data sheet for 20x SSC buffer, Mediatech, Inc.
- Molecular Cytogenetics and biology Buffers, University of Leicester
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