Harlequin may refer to:
In literature:
- Any romance novel published by Harlequin Enterprises Ltd.
- ""Repent, Harlequin!" Said the Ticktockman", a short story by Harlan Ellison.
- Harlequin Valentine, a graphic novel by Neil Gaiman.
- Harley Quinn, a character from the Batman mythos.
- The_Harlequin, a character from the Green Lantern mythos.
In entertainment:
- Harlequin, a 1980s movie.
- Arlecchino, a figure in Italian improvisional theatre.
- The Harlequins, the Dartmouth College musical theater organization.
- Harlequin, a Canadian rock band.
- The Harlequins, a racial subgroup in the wargame Warhammer 40,000.
- Harlequin, a 1990s computer game.
In other categories:
- A person suffering from Harlequin type ichthyosis, a congenital dermatological condition.
- Harlequin Inc., a defunct software company.
- The harlequin duck, an Arctic species of sea-duck.
- The Harlequins rugby union club.
- The Harlequins rugby league club.
- A clothing pattern typical of harlequins in italian improvisional art (see Arlecchino).