5-HT₄ receptor

5-Hydroxytryptamine receptor 4 is a protein that in humans is encoded by the HTR4 gene.^[5][6]

HTR4
Identifiers
Aliases	HTR4, 5-HT4, 5-HT4R, 5-HT4 receptor, 5-hydroxytryptamine receptor 4
External IDs	OMIM: 602164; MGI: 109246; HomoloGene: 20243; GeneCards: HTR4; OMA:HTR4 - orthologs
Gene ___location (Human) Chr. Chromosome 5 (human)[1] Band 5q32 Start 148,451,032 bp[1] End 148,677,235 bp[1]
Gene ___location (Mouse) Chr. Chromosome 18 (mouse)[2] Band 18|18 E1 Start 62,457,275 bp[2] End 62,629,648 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in beta cell triceps brachii muscle glutes testicle external globus pallidus vena cava vastus lateralis muscle pars reticulata pars compacta epithelium of nasopharynx Top expressed in lumbar spinal ganglion mucosa of urinary bladder transitional epithelium of urinary bladder olfactory tubercle left colon Paneth cell Region I of hippocampus proper CA3 field perirhinal cortex intestinal villus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function G protein-coupled receptor activity signal transducer activity G protein-coupled serotonin receptor activity trace-amine receptor activity identical protein binding neurotransmitter receptor activity protein binding Cellular component cytoplasm integral component of membrane endosome plasma membrane integral component of plasma membrane membrane dendrite Biological process gamma-aminobutyric acid signaling pathway G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger regulation of appetite signal transduction chemical synaptic transmission G protein-coupled serotonin receptor signaling pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3360 15562 Ensembl ENSG00000164270 ENSMUSG00000026322 UniProt Q13639 P97288 RefSeq (mRNA) NM_000870 NM_001040169 NM_001040172 NM_001040173 NM_001040174 NM_001286410 NM_199453 NM_008313 NM_001364956 NM_001364957 NM_001364958 NM_001364959 RefSeq (protein) NP_000861 NP_001035259 NP_001035262 NP_001035263 NP_001273339 NP_955525 NP_032339 NP_001351885 NP_001351886 NP_001351887 NP_001351888 Location (UCSC) Chr 5: 148.45 – 148.68 Mb Chr 18: 62.46 – 62.63 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene is a member of the family of human serotonin receptors, which are G protein-coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described, but the full-length nature of some transcript variants has not been determined.^[7]

Location

The receptor is located in the alimentary tract, urinary bladder, heart and adrenal gland as well as the central nervous system (CNS).^[8] In the CNS the receptor appears in the putamen, caudate nucleus, nucleus accumbens, globus pallidus, and substantia nigra, and to a lesser extent in the neocortex, raphe, pontine nuclei, and some areas of the thalamus. It has not been found in the cerebellum.^[9]

Isoforms

Internalization is isoform-specific.^[10]

Ligands

Several drugs that act as 5-HT₄ selective agonists have recently been introduced into use in both scientific research and clinical medicine. Some drugs that act as 5-HT₄ agonists are also active as 5-HT₃ antagonists, such as mosapride, metoclopramide, renzapride, and zacopride, and so these compounds cannot be considered highly selective. Research in this area is ongoing.^[11] Amongst these agonists prucalopride has >150-fold higher affinity for 5-HT4 receptors than for other receptors.

SB-207,145 radiolabeled with carbon-11 is used as a radioligand for 5-HT₄ in positron emission tomography pig^[12] and human^[13] studies.

Agonists

• Tropisetron^[14] – partial agonist
• BIMU-8
• Cisapride
• CJ-033,466 – partial agonist
• Felcisetrag
• ML-10302^[15]
• Mosapride
• Prucalopride
• Renzapride
• RS-67506
• RS-67333 – partial agonist
• SL65.0155 – partial agonist
• Tegaserod
• Zacopride
• Metoclopramide
• Sulpiride
• Naronapride
• Usmarapride

Antagonists

• L-lysine
• Piboserod
• GR-113,808 (1-methyl-1H-indole-3-carboxylic acid, [1-[2-[(methylsulfonyl)amino]ethyl]-4-piperidinyl]methyl ester)^[16]
• GR-125,487
• RS-39604 (1-[4-Amino-5-chloro-2-(3,5-dimethoxyphenyl)methyloxy]-3-[1-[2-methylsulphonylamino]piperidin-4-yl]propan-1-one)
• SB-203,186
• SB-204,070
• ([Methoxy-¹¹C]1-butylpiperidin-4-yl)methyl 4-amino-3-methoxybenzoate^[17]
• Chamomile (ethanol extract)^[18]

See also

• 5-HT receptor
• 5-HT₁ receptor
• 5-HT₂ receptor
• 5-HT₃ receptor
• 5-HT₅ receptor
• 5-HT₆ receptor
• 5-HT₇ receptor

References

1. GRCh38: Ensembl release 89: ENSG00000164270 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026322 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Claeysen S, Faye P, Sebben M, Lemaire S, Bockaert J, Dumuis A, Taviaux S (December 1997). "Assignment of 5-hydroxytryptamine receptor (HTR4) to human chromosome 5 bands q31→q33 by in situ hybridization" (PDF). Cytogenetics and Cell Genetics. 78 (2): 133–4. doi:10.1159/000134646. PMID 9371406.
6. Blondel O, Vandecasteele G, Gastineau M, Leclerc S, Dahmoune Y, Langlois M, Fischmeister R (August 1997). "Molecular and functional characterization of a 5-HT4 receptor cloned from human atrium". FEBS Letters. 412 (3): 465–74. Bibcode:1997FEBSL.412..465B. doi:10.1016/S0014-5793(97)00820-X. PMID 9276448. S2CID 23714426.
7. "Entrez Gene: HTR4 5-hydroxytryptamine (serotonin) receptor 4".
8. Hegde SS, Eglen RM (October 1996). "Peripheral 5-HT4 receptors". FASEB Journal. 10 (12): 1398–407. doi:10.1096/fasebj.10.12.8903510. PMID 8903510. S2CID 21596751.
9. Varnäs K, Halldin C, Pike VW, Hall H (August 2003). "Distribution of 5-HT4 receptors in the postmortem human brain--an autoradiographic study using [125I]SB 207710". European Neuropsychopharmacology. 13 (4): 228–34. doi:10.1016/S0924-977X(03)00009-9. PMID 12888181. S2CID 27945284.
10. Mnie-Filali O, Amraei MG, Benmbarek S, Archer-Lahlou E, Peñas-Cazorla R, Vilaró MT, Boye SM, Piñeyro G (March 2010). "Serotonin 4 receptor (5-HT4R) internalization is isoform-specific: effects of 5-HT and RS67333 on isoforms A and B". Cellular Signalling. 22 (3): 501–9. doi:10.1016/j.cellsig.2009.11.004. PMID 19922792.
11. Pellissier LP, Sallander J, Campillo M, Gaven F, Queffeulou E, Pillot M, Dumuis A, Claeysen S, Bockaert J, Pardo L (April 2009). "Conformational toggle switches implicated in basal constitutive and agonist-induced activated states of 5-hydroxytryptamine-4 receptors" (PDF). Molecular Pharmacology. 75 (4): 982–90. doi:10.1124/mol.108.053686. PMID 19168624. S2CID 17957338.
12. Kornum BR, Lind NM, Gillings N, Marner L, Andersen F, Knudsen GM (January 2009). "Evaluation of the novel 5-HT4 receptor PET ligand [11C]SB207145 in the Göttingen minipig". Journal of Cerebral Blood Flow and Metabolism. 29 (1): 186–96. doi:10.1038/jcbfm.2008.110. PMID 18797470.
13. Marner L, Gillings N, Comley RA, Baaré WF, Rabiner EA, Wilson AA, Houle S, Hasselbalch SG, Svarer C, Gunn RN, Laruelle M, Knudsen GM (June 2009). "Kinetic modeling of 11C-SB207145 binding to 5-HT4 receptors in the human brain in vivo". Journal of Nuclear Medicine. 50 (6): 900–8. doi:10.2967/jnumed.108.058552. PMID 19470850.
14. Costall B, Naylor RJ (November 1993). "The pharmacology of the 5-HT4 receptor". International Clinical Psychopharmacology. 8 Suppl 2 (Suppl 2): 11–18. doi:10.1097/00004850-199311002-00002. PMID 8201242. S2CID 36692776.
15. Godínez-Chaparro B, Barragán-Iglesias P, Castañeda-Corral G, Rocha-González HI, Granados-Soto V (March 2011). "Role of peripheral 5-HT(4), 5-HT(6), and 5-HT(7) receptors in development and maintenance of secondary mechanical allodynia and hyperalgesia". Pain. 152 (3): 687–97. doi:10.1016/j.pain.2010.12.020. PMID 21239110. S2CID 140204185.
16. Gale JD, Grossman CJ, Whitehead JW, Oxford AW, Bunce KT, Humphrey PP (January 1994). "GR113808: a novel, selective antagonist with high affinity at the 5-HT4 receptor". British Journal of Pharmacology. 111 (1): 332–8. doi:10.1111/j.1476-5381.1994.tb14064.x. PMC 1910004. PMID 8012715.
17. Xu R, Hong J, Morse CL, Pike VW (October 2010). "Synthesis, structure-affinity relationships, and radiolabeling of selective high-affinity 5-HT4 receptor ligands as prospective imaging probes for positron emission tomography". Journal of Medicinal Chemistry. 53 (19): 7035–47. doi:10.1021/jm100668r. PMC 2951497. PMID 20812727.
18. Simmen U, Kelber O, Okpanyi SN, Jaeggi R, Bueter B, Weiser D (2006). "Binding of STW 5 (Iberogast) and its components to intestinal 5-HT, muscarinic M3, and opioid receptors". Phytomedicine. 13 (Suppl 5): 51–5. doi:10.1016/j.phymed.2006.03.012. PMID 16973340.

Further reading

• Licht CL, 2009, Changes in the 5-HT₄ receptor in animal models of depression and antidepressant treatment, PhD thesis, University of Copenhagen.
• Ullmer C, Schmuck K, Kalkman HO, Lübbert H (August 1995). "Expression of serotonin receptor mRNAs in blood vessels". FEBS Letters. 370 (3): 215–21. Bibcode:1995FEBSL.370..215U. doi:10.1016/0014-5793(95)00828-W. PMID 7656980. S2CID 9015835.
• Blondel O, Vandecasteele G, Gastineau M, Leclerc S, Dahmoune Y, Langlois M, Fischmeister R (August 1997). "Molecular and functional characterization of a 5-HT4 receptor cloned from human atrium". FEBS Letters. 412 (3): 465–74. Bibcode:1997FEBSL.412..465B. doi:10.1016/S0014-5793(97)00820-X. PMID 9276448. S2CID 23714426.
• Van den Wyngaert I, Gommeren W, Verhasselt P, Jurzak M, Leysen J, Luyten W, Bender E (November 1997). "Cloning and expression of a human serotonin 5-HT4 receptor cDNA". Journal of Neurochemistry. 69 (5): 1810–9. doi:10.1046/j.1471-4159.1997.69051810.x. PMID 9349523. S2CID 25269904.
• Claeysen S, Faye P, Sebben M, Lemaire S, Bockaert J, Dumuis A (October 1997). "Cloning and expression of human 5-HT4S receptors. Effect of receptor density on their coupling to adenylyl cyclase" (PDF). NeuroReport. 8 (15): 3189–96. doi:10.1097/00001756-199710200-00002. PMID 9351641. S2CID 20624532.
• Blondel O, Gastineau M, Dahmoune Y, Langlois M, Fischmeister R (June 1998). "Cloning, expression, and pharmacology of four human 5-hydroxytryptamine 4 receptor isoforms produced by alternative splicing in the carboxyl terminus" (PDF). Journal of Neurochemistry. 70 (6): 2252–61. doi:10.1046/j.1471-4159.1998.70062252.x. PMID 9603189. S2CID 24203530. Archived from the original (PDF) on 10 June 2023. Retrieved 8 April 2022.
• Cichon S, Kesper K, Propping P, Nöthen MM (1998). "Assignment of the human serotonin 4 receptor gene (HTR4) to the long arm of chromosome 5 (5q31-q33)". Molecular Membrane Biology. 15 (2): 75–8. doi:10.3109/09687689809027521. PMID 9724925.
• Claeysen S, Sebben M, Becamel C, Bockaert J, Dumuis A (May 1999). "Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular ___domain" (PDF). Molecular Pharmacology. 55 (5): 910–20. doi:10.1016/S0026-895X(24)23188-6. PMID 10220570.
• Bender E, Pindon A, van Oers I, Zhang YB, Gommeren W, Verhasselt P, Jurzak M, Leysen J, Luyten W (February 2000). "Structure of the human serotonin 5-HT4 receptor gene and cloning of a novel 5-HT4 splice variant". Journal of Neurochemistry. 74 (2): 478–89. doi:10.1046/j.1471-4159.2000.740478.x. PMID 10646498. S2CID 33817190.
• Mialet J, Berque-Bestel I, Eftekhari P, Gastineau M, Giner M, Dahmoune Y, Donzeau-Gouge P, Hoebeke J, Langlois M, Sicsic S, Fischmeister R, Lezoualc'h F (February 2000). "Isolation of the serotoninergic 5-HT4(e) receptor from human heart and comparative analysis of its pharmacological profile in C6-glial and CHO cell lines". British Journal of Pharmacology. 129 (4): 771–81. doi:10.1038/sj.bjp.0703101. PMC 1571890. PMID 10683202.
• Bach T, Syversveen T, Kvingedal AM, Krobert KA, Brattelid T, Kaumann AJ, Levy FO (February 2001). "5HT4(a) and 5-HT4(b) receptors have nearly identical pharmacology and are both expressed in human atrium and ventricle". Naunyn-Schmiedeberg's Archives of Pharmacology. 363 (2): 146–60. doi:10.1007/s002100000299. PMID 11218067. S2CID 27504006.
• Medhurst AD, Lezoualc'h F, Fischmeister R, Middlemiss DN, Sanger GJ (June 2001). "Quantitative mRNA analysis of five C-terminal splice variants of the human 5-HT4 receptor in the central nervous system by TaqMan real time RT-PCR". Brain Research. Molecular Brain Research. 90 (2): 125–34. doi:10.1016/S0169-328X(01)00095-X. PMID 11406291.
• Hiroi T, Hayashi-Kobayashi N, Nagumo S, Ino M, Okawa Y, Aoba A, Matsui H (November 2001). "Identification and characterization of the human serotonin-4 receptor gene promoter". Biochemical and Biophysical Research Communications. 289 (2): 337–44. doi:10.1006/bbrc.2001.5979. PMID 11716477.
• Vilaró MT, Doménech T, Palacios JM, Mengod G (January 2002). "Cloning and characterization of a novel human 5-HT4 receptor variant that lacks the alternatively spliced carboxy terminal exon. RT-PCR distribution in human brain and periphery of multiple 5-HT4 receptor variants". Neuropharmacology. 42 (1): 60–73. doi:10.1016/S0028-3908(01)00154-X. hdl:10261/112650. PMID 11750916. S2CID 23722311.
• López-Rodríguez ML, Murcia M, Benhamú B, Olivella M, Campillo M, Pardo L (November 2001). "Computational model of the complex between GR113808 and the 5-HT4 receptor guided by site-directed mutagenesis and the crystal structure of rhodopsin". Journal of Computer-Aided Molecular Design. 15 (11): 1025–33. Bibcode:2001JCAMD..15.1025L. doi:10.1023/A:1014895611874. PMID 11989623. S2CID 23478175.
• Ohtsuki T, Ishiguro H, Detera-Wadleigh SD, Toyota T, Shimizu H, Yamada K, Yoshitsugu K, Hattori E, Yoshikawa T, Arinami T (2003). "Association between serotonin 4 receptor gene polymorphisms and bipolar disorder in Japanese case-control samples and the NIMH Genetics Initiative Bipolar Pedigrees". Molecular Psychiatry. 7 (9): 954–61. doi:10.1038/sj.mp.4001133. PMID 12399948. S2CID 21064320.
• Norum JH, Hart K, Levy FO (January 2003). "Ras-dependent ERK activation by the human G(s)-coupled serotonin receptors 5-HT4(b) and 5-HT7(a)". The Journal of Biological Chemistry. 278 (5): 3098–104. doi:10.1074/jbc.M206237200. PMID 12446729.
• Cartier D, Lihrmann I, Parmentier F, Bastard C, Bertherat J, Caron P, Kuhn JM, Lacroix A, Tabarin A, Young J, Vaudry H, Lefebvre H (January 2003). "Overexpression of serotonin4 receptors in cisapride-responsive adrenocorticotropin-independent bilateral macronodular adrenal hyperplasia causing Cushing's syndrome". The Journal of Clinical Endocrinology and Metabolism. 88 (1): 248–54. doi:10.1210/jc.2002-021107. PMID 12519861.
• Manzke T, Guenther U, Ponimaskin EG, Haller M, Dutschmann M, Schwarzacher S, Richter DW (July 2003). "5-HT4(a) receptors avert opioid-induced breathing depression without loss of analgesia". Science. 301 (5630): 226–9. Bibcode:2003Sci...301..226M. doi:10.1126/science.1084674. PMID 12855812. S2CID 13641423.

External links

• "5-HT₄". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical Pharmacology. Archived from the original on 5 June 2016. Retrieved 25 November 2008.
• Human HTR4 genome ___location and HTR4 gene details page in the UCSC Genome Browser.

This article incorporates text from the United States National Library of Medicine, which is in the public ___domain.